Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33115

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33110 and BPHYT_RS33115 are separated by 254 nucleotidesBPHYT_RS33115 and BPHYT_RS33120 are separated by 97 nucleotides BPHYT_RS33110: BPHYT_RS33110 - chloroperoxidase, at 3,004,677 to 3,005,663 _RS33110 BPHYT_RS33115: BPHYT_RS33115 - small mechanosensitive ion channel protein MscS, at 3,005,918 to 3,007,375 _RS33115 BPHYT_RS33120: BPHYT_RS33120 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,007,473 to 3,008,681 _RS33120 Position (kb) 3005 3006 3007 3008Strain fitness (log2 ratio) -1 0 1at 3005.008 kb on + strand, within BPHYT_RS33110at 3005.791 kb on - strandat 3006.403 kb on + strand, within BPHYT_RS33115at 3006.507 kb on + strand, within BPHYT_RS33115at 3006.582 kb on - strand, within BPHYT_RS33115at 3007.537 kb on + strandat 3007.673 kb on + strand, within BPHYT_RS33120at 3008.305 kb on + strand, within BPHYT_RS33120

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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3,005,008 + BPHYT_RS33110 0.34 +0.3
3,005,791 - -0.4
3,006,403 + BPHYT_RS33115 0.33 +0.1
3,006,507 + BPHYT_RS33115 0.40 +0.1
3,006,582 - BPHYT_RS33115 0.46 +1.4
3,007,537 + -0.1
3,007,673 + BPHYT_RS33120 0.17 -0.7
3,008,305 + BPHYT_RS33120 0.69 -1.0

Or see this region's nucleotide sequence