Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS27875

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS27865 and BPHYT_RS27870 are separated by 54 nucleotidesBPHYT_RS27870 and BPHYT_RS27875 are separated by 272 nucleotidesBPHYT_RS27875 and BPHYT_RS27880 are separated by 565 nucleotides BPHYT_RS27865: BPHYT_RS27865 - ATPase, at 1,813,896 to 1,816,406 _RS27865 BPHYT_RS27870: BPHYT_RS27870 - hypothetical protein, at 1,816,461 to 1,816,946 _RS27870 BPHYT_RS27875: BPHYT_RS27875 - CreA family protein, at 1,817,219 to 1,817,686 _RS27875 BPHYT_RS27880: BPHYT_RS27880 - diguanylate phosphodiesterase, at 1,818,252 to 1,821,431 _RS27880 Position (kb) 1817 1818Strain fitness (log2 ratio) -1 0 1at 1817.330 kb on + strand, within BPHYT_RS27875at 1817.331 kb on - strand, within BPHYT_RS27875at 1817.382 kb on - strand, within BPHYT_RS27875at 1817.646 kb on + strandat 1817.646 kb on + strandat 1817.649 kb on + strandat 1818.096 kb on + strandat 1818.376 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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1,817,330 + BPHYT_RS27875 0.24 -1.3
1,817,331 - BPHYT_RS27875 0.24 -0.2
1,817,382 - BPHYT_RS27875 0.35 -1.0
1,817,646 + -0.0
1,817,646 + +0.6
1,817,649 + +0.5
1,818,096 + +0.1
1,818,376 - -0.6

Or see this region's nucleotide sequence