Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23420

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23415 and BPHYT_RS23420 are separated by 390 nucleotidesBPHYT_RS23420 and BPHYT_RS23425 are separated by 88 nucleotides BPHYT_RS23415: BPHYT_RS23415 - GDSL family lipase, at 817,514 to 818,737 _RS23415 BPHYT_RS23420: BPHYT_RS23420 - acetoacetyl-CoA synthase, at 819,128 to 822,193 _RS23420 BPHYT_RS23425: BPHYT_RS23425 - mannitol dehydrogenase, at 822,282 to 823,772 _RS23425 Position (kb) 819 820 821 822 823Strain fitness (log2 ratio) -2 -1 0 1at 818.173 kb on - strand, within BPHYT_RS23415at 818.248 kb on + strand, within BPHYT_RS23415at 818.250 kb on + strand, within BPHYT_RS23415at 818.250 kb on + strand, within BPHYT_RS23415at 818.251 kb on - strand, within BPHYT_RS23415at 818.627 kb on + strandat 818.628 kb on - strandat 818.853 kb on + strandat 818.854 kb on - strandat 818.910 kb on - strandat 818.977 kb on - strandat 818.997 kb on + strandat 819.100 kb on - strandat 819.168 kb on - strandat 819.184 kb on - strandat 819.434 kb on + strandat 819.435 kb on - strand, within BPHYT_RS23420at 820.175 kb on + strand, within BPHYT_RS23420at 821.840 kb on - strand, within BPHYT_RS23420

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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818,173 - BPHYT_RS23415 0.54 -0.6
818,248 + BPHYT_RS23415 0.60 -0.8
818,250 + BPHYT_RS23415 0.60 -0.6
818,250 + BPHYT_RS23415 0.60 -1.7
818,251 - BPHYT_RS23415 0.60 +0.4
818,627 + -2.7
818,628 - +0.5
818,853 + -1.1
818,854 - +0.1
818,910 - +0.0
818,977 - -0.2
818,997 + -0.4
819,100 - -0.6
819,168 - -0.2
819,184 - +0.0
819,434 + +0.4
819,435 - BPHYT_RS23420 0.10 +0.8
820,175 + BPHYT_RS23420 0.34 -0.3
821,840 - BPHYT_RS23420 0.88 -0.2

Or see this region's nucleotide sequence