Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21080 and BPHYT_RS21085 overlap by 4 nucleotides BPHYT_RS21085 and BPHYT_RS21090 are separated by 19 nucleotides BPHYT_RS21090 and BPHYT_RS21095 are separated by 335 nucleotides
BPHYT_RS21080: BPHYT_RS21080 - (2Fe-2S)-binding protein, at 290,385 to 291,500
_RS21080
BPHYT_RS21085: BPHYT_RS21085 - hypothetical protein, at 291,497 to 291,706
_RS21085
BPHYT_RS21090: BPHYT_RS21090 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 291,726 to 292,955
_RS21090
BPHYT_RS21095: BPHYT_RS21095 - omega amino acid--pyruvate aminotransferase, at 293,291 to 294,607
_RS21095
Position (kb) 291
292
293 Strain fitness (log2 ratio) -2
-1
0
1
2 at 290.764 kb on + strand, within BPHYT_RS21080 at 290.862 kb on + strand, within BPHYT_RS21080 at 290.923 kb on - strand, within BPHYT_RS21080 at 291.315 kb on - strand, within BPHYT_RS21080 at 291.867 kb on + strand, within BPHYT_RS21090 at 291.930 kb on + strand, within BPHYT_RS21090 at 291.932 kb on + strand, within BPHYT_RS21090 at 291.972 kb on + strand, within BPHYT_RS21090 at 291.973 kb on - strand, within BPHYT_RS21090 at 292.058 kb on + strand, within BPHYT_RS21090 at 292.059 kb on - strand, within BPHYT_RS21090 at 293.396 kb on + strand at 293.397 kb on - strand at 293.651 kb on - strand, within BPHYT_RS21095 at 293.651 kb on - strand, within BPHYT_RS21095
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 290,764 + BPHYT_RS21080 0.34 -1.3 290,862 + BPHYT_RS21080 0.43 +0.0 290,923 - BPHYT_RS21080 0.48 +1.9 291,315 - BPHYT_RS21080 0.83 -1.6 291,867 + BPHYT_RS21090 0.11 +0.1 291,930 + BPHYT_RS21090 0.17 -0.9 291,932 + BPHYT_RS21090 0.17 -1.6 291,972 + BPHYT_RS21090 0.20 +0.1 291,973 - BPHYT_RS21090 0.20 +0.4 292,058 + BPHYT_RS21090 0.27 +0.1 292,059 - BPHYT_RS21090 0.27 +1.0 293,396 + -0.4 293,397 - -0.2 293,651 - BPHYT_RS21095 0.27 -1.9 293,651 - BPHYT_RS21095 0.27 -0.7
Or see this region's nucleotide sequence