Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20050 and BPHYT_RS20055 are separated by 342 nucleotides BPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotides BPHYT_RS20060 and BPHYT_RS20065 overlap by 1 nucleotides BPHYT_RS20065 and BPHYT_RS20070 are separated by 81 nucleotides
BPHYT_RS20050: BPHYT_RS20050 - hypothetical protein, at 43,294 to 43,542
_RS20050
BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202
_RS20055
BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122
_RS20060
BPHYT_RS20065: BPHYT_RS20065 - hypothetical protein, at 45,122 to 45,604
_RS20065
BPHYT_RS20070: BPHYT_RS20070 - hypothetical protein, at 45,686 to 47,119
_RS20070
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1
2 at 43.776 kb on + strand at 43.843 kb on - strand at 43.886 kb on + strand at 44.069 kb on + strand, within BPHYT_RS20055 at 44.069 kb on + strand, within BPHYT_RS20055 at 44.236 kb on + strand at 44.360 kb on - strand at 44.639 kb on - strand, within BPHYT_RS20060 at 45.145 kb on + strand at 45.418 kb on - strand, within BPHYT_RS20065 at 45.630 kb on + strand at 45.631 kb on - strand at 45.646 kb on - strand at 45.661 kb on - strand at 46.098 kb on - strand, within BPHYT_RS20070
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 43,776 + +0.7 43,843 - -1.4 43,886 + -2.6 44,069 + BPHYT_RS20055 0.58 +1.4 44,069 + BPHYT_RS20055 0.58 -0.0 44,236 + -0.1 44,360 - +0.4 44,639 - BPHYT_RS20060 0.30 -0.5 45,145 + +1.9 45,418 - BPHYT_RS20065 0.61 -1.8 45,630 + -0.3 45,631 - -1.4 45,646 - +0.6 45,661 - +0.0 46,098 - BPHYT_RS20070 0.29 +0.4
Or see this region's nucleotide sequence