Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS04055

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS04050 and BPHYT_RS04055 are separated by 330 nucleotidesBPHYT_RS04055 and BPHYT_RS04060 overlap by 1 nucleotides BPHYT_RS04050: BPHYT_RS04050 - group 1 glycosyl transferase, at 923,700 to 924,905 _RS04050 BPHYT_RS04055: BPHYT_RS04055 - PST family polysaccharide export protein, at 925,236 to 926,489 _RS04055 BPHYT_RS04060: BPHYT_RS04060 - glycosyl transferase, at 926,489 to 927,562 _RS04060 Position (kb) 925 926 927Strain fitness (log2 ratio) -2 -1 0 1 2at 924.550 kb on + strand, within BPHYT_RS04050at 924.877 kb on + strandat 924.878 kb on - strandat 924.878 kb on - strandat 924.920 kb on + strandat 924.986 kb on + strandat 924.991 kb on - strandat 925.148 kb on + strandat 925.149 kb on - strandat 925.659 kb on + strand, within BPHYT_RS04055at 925.660 kb on - strand, within BPHYT_RS04055at 925.663 kb on - strand, within BPHYT_RS04055at 925.755 kb on + strand, within BPHYT_RS04055at 925.996 kb on - strand, within BPHYT_RS04055at 926.127 kb on + strand, within BPHYT_RS04055at 926.458 kb on + strandat 926.813 kb on + strand, within BPHYT_RS04060at 927.116 kb on + strand, within BPHYT_RS04060at 927.117 kb on - strand, within BPHYT_RS04060at 927.144 kb on - strand, within BPHYT_RS04060

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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924,550 + BPHYT_RS04050 0.70 +0.0
924,877 + +2.1
924,878 - +0.6
924,878 - +0.6
924,920 + -1.9
924,986 + -0.0
924,991 - -1.4
925,148 + +0.0
925,149 - -2.0
925,659 + BPHYT_RS04055 0.34 -1.1
925,660 - BPHYT_RS04055 0.34 -1.5
925,663 - BPHYT_RS04055 0.34 -1.1
925,755 + BPHYT_RS04055 0.41 -0.1
925,996 - BPHYT_RS04055 0.61 -1.3
926,127 + BPHYT_RS04055 0.71 +0.5
926,458 + -0.2
926,813 + BPHYT_RS04060 0.30 -2.0
927,116 + BPHYT_RS04060 0.58 +0.5
927,117 - BPHYT_RS04060 0.58 -1.2
927,144 - BPHYT_RS04060 0.61 -0.9

Or see this region's nucleotide sequence