Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02890 and BPHYT_RS02895 are separated by 127 nucleotides BPHYT_RS02895 and BPHYT_RS02900 are separated by 337 nucleotides BPHYT_RS02900 and BPHYT_RS02905 are separated by 170 nucleotides
BPHYT_RS02890: BPHYT_RS02890 - HPr kinase/phosphorylase, at 643,976 to 644,944
_RS02890
BPHYT_RS02895: BPHYT_RS02895 - PTS sugar transporter subunit IIA, at 645,072 to 645,527
_RS02895
BPHYT_RS02900: BPHYT_RS02900 - ribosome hibernation promoting factor, at 645,865 to 646,221
_RS02900
BPHYT_RS02905: BPHYT_RS02905 - RNA polymerase subunit sigma-54, at 646,392 to 647,903
_RS02905
Position (kb) 645
646
647 Strain fitness (log2 ratio) -1
0
1
2
3 at 645.026 kb on - strand at 645.663 kb on + strand at 645.691 kb on + strand at 645.691 kb on + strand at 645.692 kb on - strand at 645.692 kb on - strand at 645.692 kb on - strand at 645.692 kb on - strand at 645.693 kb on + strand at 645.693 kb on + strand at 645.693 kb on + strand at 645.694 kb on - strand at 645.694 kb on - strand at 645.736 kb on + strand at 645.884 kb on - strand at 645.902 kb on + strand, within BPHYT_RS02900 at 645.903 kb on - strand, within BPHYT_RS02900 at 645.996 kb on - strand, within BPHYT_RS02900 at 646.139 kb on - strand, within BPHYT_RS02900 at 646.186 kb on + strand at 646.221 kb on + strand at 646.221 kb on + strand at 646.237 kb on + strand at 646.237 kb on + strand at 646.238 kb on - strand at 646.238 kb on - strand at 646.265 kb on + strand at 646.266 kb on - strand at 646.266 kb on - strand at 646.266 kb on - strand at 646.365 kb on + strand at 646.393 kb on + strand at 646.394 kb on - strand at 646.442 kb on - strand at 646.455 kb on - strand at 646.540 kb on - strand at 646.553 kb on + strand, within BPHYT_RS02905 at 646.554 kb on - strand, within BPHYT_RS02905 at 646.621 kb on + strand, within BPHYT_RS02905 at 646.625 kb on + strand, within BPHYT_RS02905 at 646.626 kb on - strand, within BPHYT_RS02905 at 646.657 kb on + strand, within BPHYT_RS02905 at 646.657 kb on + strand at 646.658 kb on - strand, within BPHYT_RS02905 at 646.658 kb on - strand, within BPHYT_RS02905 at 646.658 kb on - strand, within BPHYT_RS02905 at 646.658 kb on - strand, within BPHYT_RS02905 at 646.706 kb on + strand, within BPHYT_RS02905 at 646.707 kb on - strand, within BPHYT_RS02905 at 646.707 kb on - strand, within BPHYT_RS02905 at 646.707 kb on - strand, within BPHYT_RS02905 at 646.895 kb on + strand, within BPHYT_RS02905 at 646.916 kb on - strand, within BPHYT_RS02905 at 646.916 kb on - strand, within BPHYT_RS02905 at 646.925 kb on + strand, within BPHYT_RS02905 at 646.929 kb on - strand, within BPHYT_RS02905 at 646.929 kb on - strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.011 kb on + strand, within BPHYT_RS02905 at 647.012 kb on - strand, within BPHYT_RS02905 at 647.012 kb on - strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.070 kb on + strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.071 kb on - strand, within BPHYT_RS02905 at 647.172 kb on + strand, within BPHYT_RS02905 at 647.189 kb on + strand, within BPHYT_RS02905
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 645,026 - +0.4 645,663 + +2.3 645,691 + +0.9 645,691 + +0.3 645,692 - -1.0 645,692 - -0.5 645,692 - -1.6 645,692 - +0.1 645,693 + +0.8 645,693 + +0.2 645,693 + +1.7 645,694 - -0.2 645,694 - +2.3 645,736 + +1.1 645,884 - +0.3 645,902 + BPHYT_RS02900 0.10 +0.7 645,903 - BPHYT_RS02900 0.11 +1.4 645,996 - BPHYT_RS02900 0.37 +1.6 646,139 - BPHYT_RS02900 0.77 +0.1 646,186 + +0.0 646,221 + +0.9 646,221 + +0.3 646,237 + -1.6 646,237 + -0.4 646,238 - -0.5 646,238 - -0.9 646,265 + -0.0 646,266 - +0.4 646,266 - +1.8 646,266 - -0.4 646,365 + -0.6 646,393 + -0.3 646,394 - +1.3 646,442 - +1.3 646,455 - +1.3 646,540 - +1.8 646,553 + BPHYT_RS02905 0.11 +2.8 646,554 - BPHYT_RS02905 0.11 +1.7 646,621 + BPHYT_RS02905 0.15 +2.2 646,625 + BPHYT_RS02905 0.15 +2.2 646,626 - BPHYT_RS02905 0.15 +1.7 646,657 + BPHYT_RS02905 0.18 +1.4 646,657 + +3.0 646,658 - BPHYT_RS02905 0.18 +1.6 646,658 - BPHYT_RS02905 0.18 +1.6 646,658 - BPHYT_RS02905 0.18 +1.6 646,658 - BPHYT_RS02905 0.18 +1.7 646,706 + BPHYT_RS02905 0.21 +1.7 646,707 - BPHYT_RS02905 0.21 +1.9 646,707 - BPHYT_RS02905 0.21 +1.5 646,707 - BPHYT_RS02905 0.21 +1.9 646,895 + BPHYT_RS02905 0.33 +1.3 646,916 - BPHYT_RS02905 0.35 +1.5 646,916 - BPHYT_RS02905 0.35 +1.7 646,925 + BPHYT_RS02905 0.35 +2.0 646,929 - BPHYT_RS02905 0.36 +1.7 646,929 - BPHYT_RS02905 0.36 +1.6 647,011 + BPHYT_RS02905 0.41 +2.4 647,011 + BPHYT_RS02905 0.41 +1.7 647,011 + BPHYT_RS02905 0.41 +1.9 647,011 + BPHYT_RS02905 0.41 +1.9 647,011 + BPHYT_RS02905 0.41 +1.6 647,012 - BPHYT_RS02905 0.41 +1.8 647,012 - BPHYT_RS02905 0.41 +1.8 647,070 + BPHYT_RS02905 0.45 +2.5 647,070 + BPHYT_RS02905 0.45 +2.5 647,070 + BPHYT_RS02905 0.45 +1.6 647,070 + BPHYT_RS02905 0.45 +1.7 647,071 - BPHYT_RS02905 0.45 +2.0 647,071 - BPHYT_RS02905 0.45 +1.8 647,071 - BPHYT_RS02905 0.45 +1.8 647,071 - BPHYT_RS02905 0.45 +1.8 647,071 - BPHYT_RS02905 0.45 +2.3 647,071 - BPHYT_RS02905 0.45 +1.7 647,172 + BPHYT_RS02905 0.52 +1.8 647,189 + BPHYT_RS02905 0.53 +3.1
Or see this region's nucleotide sequence