Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00155 and BPHYT_RS00160 are separated by 124 nucleotides BPHYT_RS00160 and BPHYT_RS00165 are separated by 131 nucleotides BPHYT_RS00165 and BPHYT_RS00170 are separated by 22 nucleotides
BPHYT_RS00155: BPHYT_RS00155 - aminotransferase, at 41,982 to 43,136
_RS00155
BPHYT_RS00160: BPHYT_RS00160 - ABC transporter substrate-binding protein, at 43,261 to 44,049
_RS00160
BPHYT_RS00165: BPHYT_RS00165 - AraC family transcriptional regulator, at 44,181 to 45,026
_RS00165
BPHYT_RS00170: BPHYT_RS00170 - lysine transporter LysE, at 45,049 to 45,636
_RS00170
Position (kb) 43
44
45 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 42.291 kb on + strand, within BPHYT_RS00155 at 42.337 kb on - strand, within BPHYT_RS00155 at 42.337 kb on - strand, within BPHYT_RS00155 at 42.360 kb on + strand, within BPHYT_RS00155 at 42.361 kb on - strand, within BPHYT_RS00155 at 42.591 kb on + strand, within BPHYT_RS00155 at 42.739 kb on + strand, within BPHYT_RS00155 at 42.801 kb on + strand, within BPHYT_RS00155 at 42.838 kb on - strand, within BPHYT_RS00155 at 42.838 kb on - strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 42.858 kb on + strand, within BPHYT_RS00155 at 43.044 kb on - strand at 43.052 kb on + strand at 43.140 kb on + strand at 43.141 kb on - strand at 43.173 kb on + strand at 43.173 kb on + strand at 43.185 kb on + strand at 43.185 kb on + strand at 43.185 kb on + strand at 43.456 kb on - strand, within BPHYT_RS00160 at 43.768 kb on + strand, within BPHYT_RS00160 at 43.768 kb on + strand, within BPHYT_RS00160 at 44.047 kb on + strand at 44.048 kb on - strand at 44.048 kb on - strand at 44.059 kb on + strand at 44.059 kb on + strand at 44.129 kb on + strand at 44.130 kb on - strand at 44.133 kb on + strand at 44.155 kb on + strand at 44.163 kb on + strand at 44.178 kb on + strand at 44.302 kb on - strand, within BPHYT_RS00165 at 44.362 kb on - strand, within BPHYT_RS00165 at 44.847 kb on + strand, within BPHYT_RS00165 at 44.868 kb on + strand, within BPHYT_RS00165 at 44.868 kb on + strand, within BPHYT_RS00165 at 44.893 kb on - strand, within BPHYT_RS00165 at 44.893 kb on - strand, within BPHYT_RS00165 at 45.016 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 42,291 + BPHYT_RS00155 0.27 +1.5 42,337 - BPHYT_RS00155 0.31 -0.5 42,337 - BPHYT_RS00155 0.31 -0.6 42,360 + BPHYT_RS00155 0.33 +0.1 42,361 - BPHYT_RS00155 0.33 -0.4 42,591 + BPHYT_RS00155 0.53 -0.1 42,739 + BPHYT_RS00155 0.66 +0.4 42,801 + BPHYT_RS00155 0.71 -0.3 42,838 - BPHYT_RS00155 0.74 -0.6 42,838 - BPHYT_RS00155 0.74 +1.4 42,858 + BPHYT_RS00155 0.76 +0.0 42,858 + BPHYT_RS00155 0.76 -0.3 42,858 + BPHYT_RS00155 0.76 -0.3 43,044 - -0.2 43,052 + -0.5 43,140 + -1.0 43,141 - -0.0 43,173 + +0.0 43,173 + +0.2 43,185 + -0.6 43,185 + +0.2 43,185 + +0.7 43,456 - BPHYT_RS00160 0.25 -0.5 43,768 + BPHYT_RS00160 0.64 -0.3 43,768 + BPHYT_RS00160 0.64 -1.0 44,047 + -2.5 44,048 - -0.6 44,048 - +0.0 44,059 + -0.7 44,059 + -1.9 44,129 + -0.7 44,130 - -0.9 44,133 + -0.5 44,155 + -0.2 44,163 + +0.3 44,178 + -1.1 44,302 - BPHYT_RS00165 0.14 -1.2 44,362 - BPHYT_RS00165 0.21 -0.8 44,847 + BPHYT_RS00165 0.79 +0.2 44,868 + BPHYT_RS00165 0.81 -3.0 44,868 + BPHYT_RS00165 0.81 +0.4 44,893 - BPHYT_RS00165 0.84 -2.8 44,893 - BPHYT_RS00165 0.84 -0.4 45,016 - -1.5
Or see this region's nucleotide sequence