Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32530

Experiment: Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32525 and BPHYT_RS32530 are separated by 250 nucleotidesBPHYT_RS32530 and BPHYT_RS32535 are separated by 33 nucleotidesBPHYT_RS32535 and BPHYT_RS32540 are separated by 187 nucleotidesBPHYT_RS32540 and BPHYT_RS32545 are separated by 152 nucleotides BPHYT_RS32525: BPHYT_RS32525 - hypothetical protein, at 2,869,221 to 2,869,685 _RS32525 BPHYT_RS32530: BPHYT_RS32530 - transposase, at 2,869,936 to 2,871,492 _RS32530 BPHYT_RS32535: BPHYT_RS32535 - transposase, at 2,871,526 to 2,871,876 _RS32535 BPHYT_RS32540: BPHYT_RS32540 - hypothetical protein, at 2,872,064 to 2,872,321 _RS32540 BPHYT_RS32545: BPHYT_RS32545 - hypothetical protein, at 2,872,474 to 2,872,689 _RS32545 Position (kb) 2869 2870 2871 2872Strain fitness (log2 ratio) -2 -1 0 1 2at 2869.140 kb on + strandat 2869.186 kb on + strandat 2869.206 kb on - strandat 2869.232 kb on + strandat 2869.233 kb on - strandat 2869.233 kb on - strandat 2869.340 kb on - strand, within BPHYT_RS32525at 2869.408 kb on + strand, within BPHYT_RS32525at 2869.641 kb on - strandat 2869.670 kb on - strandat 2869.670 kb on - strandat 2869.673 kb on + strandat 2869.866 kb on - strandat 2869.889 kb on + strandat 2869.900 kb on + strandat 2869.979 kb on + strandat 2870.030 kb on + strandat 2870.241 kb on - strand, within BPHYT_RS32530at 2870.558 kb on + strand, within BPHYT_RS32530at 2870.575 kb on + strand, within BPHYT_RS32530at 2870.640 kb on - strand, within BPHYT_RS32530at 2870.690 kb on + strand, within BPHYT_RS32530at 2870.690 kb on + strand, within BPHYT_RS32530at 2870.919 kb on + strand, within BPHYT_RS32530at 2870.919 kb on + strand, within BPHYT_RS32530at 2871.366 kb on - strandat 2871.502 kb on - strandat 2871.592 kb on + strand, within BPHYT_RS32535at 2871.646 kb on + strand, within BPHYT_RS32535at 2871.646 kb on + strand, within BPHYT_RS32535at 2871.746 kb on + strand, within BPHYT_RS32535at 2871.845 kb on + strandat 2871.845 kb on + strandat 2871.846 kb on - strandat 2871.846 kb on - strandat 2872.082 kb on + strandat 2872.267 kb on - strand, within BPHYT_RS32540at 2872.370 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
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2,869,140 + +1.2
2,869,186 + +0.3
2,869,206 - -1.0
2,869,232 + -0.2
2,869,233 - -0.1
2,869,233 - +1.0
2,869,340 - BPHYT_RS32525 0.26 -1.2
2,869,408 + BPHYT_RS32525 0.40 -1.0
2,869,641 - +0.5
2,869,670 - +2.6
2,869,670 - -1.1
2,869,673 + +0.3
2,869,866 - -1.6
2,869,889 + -1.6
2,869,900 + -0.2
2,869,979 + +2.1
2,870,030 + +0.2
2,870,241 - BPHYT_RS32530 0.20 +0.5
2,870,558 + BPHYT_RS32530 0.40 -1.1
2,870,575 + BPHYT_RS32530 0.41 -1.0
2,870,640 - BPHYT_RS32530 0.45 -1.0
2,870,690 + BPHYT_RS32530 0.48 -1.0
2,870,690 + BPHYT_RS32530 0.48 +1.0
2,870,919 + BPHYT_RS32530 0.63 -0.2
2,870,919 + BPHYT_RS32530 0.63 +0.6
2,871,366 - +0.3
2,871,502 - -1.4
2,871,592 + BPHYT_RS32535 0.19 +0.7
2,871,646 + BPHYT_RS32535 0.34 -0.8
2,871,646 + BPHYT_RS32535 0.34 -0.6
2,871,746 + BPHYT_RS32535 0.63 -0.9
2,871,845 + -0.3
2,871,845 + -0.6
2,871,846 - -0.7
2,871,846 - -0.5
2,872,082 + -2.4
2,872,267 - BPHYT_RS32540 0.79 -0.9
2,872,370 + -2.6

Or see this region's nucleotide sequence