Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20110

Experiment: Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20105 and BPHYT_RS20110 are separated by 66 nucleotidesBPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotidesBPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotides BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419 _RS20105 BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868 _RS20110 BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592 _RS20115 BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899 _RS20120 Position (kb) 54 55 56Strain fitness (log2 ratio) -2 -1 0 1 2 3at 53.895 kb on + strand, within BPHYT_RS20105at 53.896 kb on - strand, within BPHYT_RS20105at 54.035 kb on + strand, within BPHYT_RS20105at 54.036 kb on - strand, within BPHYT_RS20105at 54.421 kb on - strandat 54.738 kb on + strand, within BPHYT_RS20110at 54.979 kb on + strand, within BPHYT_RS20110at 55.023 kb on + strand, within BPHYT_RS20110at 55.628 kb on + strand, within BPHYT_RS20110at 55.877 kb on + strandat 56.026 kb on + strand, within BPHYT_RS20115at 56.469 kb on + strand, within BPHYT_RS20115at 56.475 kb on + strand, within BPHYT_RS20115at 56.625 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
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53,895 + BPHYT_RS20105 0.50 +2.4
53,896 - BPHYT_RS20105 0.50 +0.1
54,035 + BPHYT_RS20105 0.63 +0.3
54,036 - BPHYT_RS20105 0.63 -1.5
54,421 - -0.9
54,738 + BPHYT_RS20110 0.18 -1.0
54,979 + BPHYT_RS20110 0.36 -2.0
55,023 + BPHYT_RS20110 0.39 +0.4
55,628 + BPHYT_RS20110 0.83 -1.0
55,877 + -1.2
56,026 + BPHYT_RS20115 0.19 +2.1
56,469 + BPHYT_RS20115 0.82 +3.5
56,475 + BPHYT_RS20115 0.83 +0.4
56,625 - +0.2

Or see this region's nucleotide sequence