Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12025

Experiment: Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12020 and BPHYT_RS12025 are separated by 272 nucleotidesBPHYT_RS12025 and BPHYT_RS12030 are separated by 41 nucleotidesBPHYT_RS12030 and BPHYT_RS12035 overlap by 4 nucleotidesBPHYT_RS12035 and BPHYT_RS12040 are separated by 100 nucleotides BPHYT_RS12020: BPHYT_RS12020 - inosine 5'-monophosphate dehydrogenase, at 2,740,709 to 2,742,169 _RS12020 BPHYT_RS12025: BPHYT_RS12025 - multidrug DMT transporter permease, at 2,742,442 to 2,743,419 _RS12025 BPHYT_RS12030: BPHYT_RS12030 - protein RnfH, at 2,743,461 to 2,743,793 _RS12030 BPHYT_RS12035: BPHYT_RS12035 - cyclase, at 2,743,790 to 2,744,227 _RS12035 BPHYT_RS12040: BPHYT_RS12040 - single-stranded DNA-binding protein, at 2,744,328 to 2,744,774 _RS12040 Position (kb) 2742 2743 2744Strain fitness (log2 ratio) -3 -2 -1 0 1at 2742.231 kb on - strandat 2742.284 kb on - strandat 2742.403 kb on - strandat 2742.460 kb on + strandat 2742.460 kb on + strandat 2742.467 kb on + strandat 2742.482 kb on + strandat 2742.553 kb on + strand, within BPHYT_RS12025at 2742.562 kb on + strand, within BPHYT_RS12025at 2742.677 kb on - strand, within BPHYT_RS12025at 2742.677 kb on - strand, within BPHYT_RS12025at 2742.677 kb on - strand, within BPHYT_RS12025at 2742.679 kb on + strand, within BPHYT_RS12025at 2742.679 kb on + strand, within BPHYT_RS12025at 2742.679 kb on + strand, within BPHYT_RS12025at 2742.679 kb on + strand, within BPHYT_RS12025at 2742.865 kb on - strand, within BPHYT_RS12025at 2742.973 kb on + strand, within BPHYT_RS12025at 2743.261 kb on + strand, within BPHYT_RS12025at 2743.368 kb on + strandat 2743.571 kb on - strand, within BPHYT_RS12030at 2743.762 kb on + strandat 2743.803 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
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2,742,231 - +0.6
2,742,284 - +1.2
2,742,403 - -0.1
2,742,460 + -0.1
2,742,460 + -1.5
2,742,467 + -2.3
2,742,482 + -0.3
2,742,553 + BPHYT_RS12025 0.11 +0.4
2,742,562 + BPHYT_RS12025 0.12 -0.3
2,742,677 - BPHYT_RS12025 0.24 -1.7
2,742,677 - BPHYT_RS12025 0.24 -1.3
2,742,677 - BPHYT_RS12025 0.24 +0.3
2,742,679 + BPHYT_RS12025 0.24 -3.0
2,742,679 + BPHYT_RS12025 0.24 -0.7
2,742,679 + BPHYT_RS12025 0.24 +1.0
2,742,679 + BPHYT_RS12025 0.24 -1.2
2,742,865 - BPHYT_RS12025 0.43 -0.4
2,742,973 + BPHYT_RS12025 0.54 -1.4
2,743,261 + BPHYT_RS12025 0.84 +0.0
2,743,368 + -0.6
2,743,571 - BPHYT_RS12030 0.33 -1.5
2,743,762 + +1.0
2,743,803 + -1.0

Or see this region's nucleotide sequence