Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19275

Experiment: KLEB28 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19270 and BWI76_RS19275 overlap by 4 nucleotidesBWI76_RS19275 and BWI76_RS19280 are separated by 292 nucleotides BWI76_RS19270: BWI76_RS19270 - two-component sensor histidine kinase, at 4,013,956 to 4,015,491 _RS19270 BWI76_RS19275: BWI76_RS19275 - DNA-binding response regulator, at 4,015,488 to 4,016,210 _RS19275 BWI76_RS19280: BWI76_RS19280 - U32 family peptidase, at 4,016,503 to 4,017,864 _RS19280 Position (kb) 4015 4016 4017Strain fitness (log2 ratio) -2 -1 0 1at 4014.604 kb on - strand, within BWI76_RS19270at 4014.785 kb on + strand, within BWI76_RS19270at 4015.365 kb on - strandat 4015.803 kb on + strand, within BWI76_RS19275at 4015.817 kb on + strand, within BWI76_RS19275at 4015.867 kb on + strand, within BWI76_RS19275at 4016.167 kb on + strandat 4016.168 kb on - strandat 4016.435 kb on - strandat 4016.454 kb on - strandat 4016.481 kb on - strandat 4016.581 kb on + strandat 4016.685 kb on - strand, within BWI76_RS19280at 4016.720 kb on - strand, within BWI76_RS19280at 4016.856 kb on + strand, within BWI76_RS19280at 4016.856 kb on + strand, within BWI76_RS19280at 4017.009 kb on + strand, within BWI76_RS19280

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB28 MOI 0.1
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4,014,604 - BWI76_RS19270 0.42 -0.1
4,014,785 + BWI76_RS19270 0.54 +0.4
4,015,365 - +1.3
4,015,803 + BWI76_RS19275 0.44 -2.4
4,015,817 + BWI76_RS19275 0.46 -0.6
4,015,867 + BWI76_RS19275 0.52 -0.4
4,016,167 + -1.1
4,016,168 - -1.2
4,016,435 - -0.0
4,016,454 - -0.1
4,016,481 - +0.3
4,016,581 + -0.5
4,016,685 - BWI76_RS19280 0.13 -0.0
4,016,720 - BWI76_RS19280 0.16 -0.4
4,016,856 + BWI76_RS19280 0.26 +0.1
4,016,856 + BWI76_RS19280 0.26 +1.1
4,017,009 + BWI76_RS19280 0.37 +0.4

Or see this region's nucleotide sequence