Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17920

Experiment: KLEB28 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17905 and BWI76_RS17910 are separated by 106 nucleotidesBWI76_RS17910 and BWI76_RS17915 are separated by 96 nucleotidesBWI76_RS17915 and BWI76_RS17920 are separated by 3 nucleotidesBWI76_RS17920 and BWI76_RS17925 are separated by 60 nucleotides BWI76_RS17905: BWI76_RS17905 - hypothetical protein, at 3,724,326 to 3,724,784 _RS17905 BWI76_RS17910: BWI76_RS17910 - hypothetical protein, at 3,724,891 to 3,725,112 _RS17910 BWI76_RS17915: BWI76_RS17915 - membrane protein, at 3,725,209 to 3,725,775 _RS17915 BWI76_RS17920: BWI76_RS17920 - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 3,725,779 to 3,726,573 _RS17920 BWI76_RS17925: BWI76_RS17925 - peptidoglycan synthase, at 3,726,634 to 3,728,379 _RS17925 Position (kb) 3725 3726 3727Strain fitness (log2 ratio) -3 -2 -1 0 1at 3724.801 kb on + strandat 3724.806 kb on + strandat 3724.806 kb on + strandat 3724.806 kb on + strandat 3724.806 kb on + strandat 3724.835 kb on - strandat 3724.840 kb on - strandat 3724.980 kb on + strand, within BWI76_RS17910at 3725.035 kb on - strand, within BWI76_RS17910at 3725.063 kb on - strand, within BWI76_RS17910at 3725.069 kb on + strand, within BWI76_RS17910at 3725.074 kb on + strand, within BWI76_RS17910at 3725.077 kb on - strand, within BWI76_RS17910at 3725.077 kb on - strand, within BWI76_RS17910at 3725.077 kb on - strand, within BWI76_RS17910at 3725.089 kb on + strand, within BWI76_RS17910at 3725.099 kb on + strandat 3725.099 kb on + strandat 3725.108 kb on - strandat 3725.109 kb on - strandat 3725.110 kb on + strandat 3725.118 kb on - strandat 3725.118 kb on - strandat 3725.189 kb on + strandat 3725.326 kb on + strand, within BWI76_RS17915at 3725.334 kb on - strand, within BWI76_RS17915at 3725.334 kb on - strand, within BWI76_RS17915at 3725.576 kb on + strand, within BWI76_RS17915at 3725.623 kb on + strand, within BWI76_RS17915at 3725.631 kb on - strand, within BWI76_RS17915at 3725.728 kb on - strandat 3725.743 kb on + strandat 3725.926 kb on - strand, within BWI76_RS17920at 3725.979 kb on + strand, within BWI76_RS17920at 3726.603 kb on + strandat 3726.611 kb on - strandat 3726.993 kb on - strand, within BWI76_RS17925at 3727.153 kb on + strand, within BWI76_RS17925at 3727.353 kb on + strand, within BWI76_RS17925at 3727.368 kb on - strand, within BWI76_RS17925

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB28 MOI 0.1
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3,724,801 + +0.4
3,724,806 + +0.0
3,724,806 + -0.2
3,724,806 + +0.1
3,724,806 + -2.9
3,724,835 - +0.6
3,724,840 - -0.9
3,724,980 + BWI76_RS17910 0.40 +0.3
3,725,035 - BWI76_RS17910 0.65 +0.9
3,725,063 - BWI76_RS17910 0.77 +1.1
3,725,069 + BWI76_RS17910 0.80 +0.0
3,725,074 + BWI76_RS17910 0.82 +0.6
3,725,077 - BWI76_RS17910 0.84 -0.4
3,725,077 - BWI76_RS17910 0.84 -1.5
3,725,077 - BWI76_RS17910 0.84 -0.0
3,725,089 + BWI76_RS17910 0.89 +0.4
3,725,099 + -0.7
3,725,099 + +0.3
3,725,108 - -0.4
3,725,109 - -0.8
3,725,110 + +0.0
3,725,118 - -0.0
3,725,118 - -0.4
3,725,189 + -0.6
3,725,326 + BWI76_RS17915 0.21 +0.3
3,725,334 - BWI76_RS17915 0.22 +0.7
3,725,334 - BWI76_RS17915 0.22 +0.1
3,725,576 + BWI76_RS17915 0.65 +0.2
3,725,623 + BWI76_RS17915 0.73 +0.7
3,725,631 - BWI76_RS17915 0.74 -0.1
3,725,728 - -0.0
3,725,743 + -1.1
3,725,926 - BWI76_RS17920 0.18 -0.3
3,725,979 + BWI76_RS17920 0.25 -1.6
3,726,603 + +0.7
3,726,611 - -0.1
3,726,993 - BWI76_RS17925 0.21 -0.9
3,727,153 + BWI76_RS17925 0.30 -1.4
3,727,353 + BWI76_RS17925 0.41 +0.0
3,727,368 - BWI76_RS17925 0.42 -0.2

Or see this region's nucleotide sequence