Experiment: KLEB28 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15085 and BWI76_RS15090 overlap by 11 nucleotides BWI76_RS15090 and BWI76_RS15095 are separated by 18 nucleotides BWI76_RS15095 and BWI76_RS15100 are separated by 29 nucleotides
BWI76_RS15085: BWI76_RS15085 - 3-deoxy-D-arabinose-heptulosonic-7-phosphate synthase, at 3,160,014 to 3,161,183
_RS15085
BWI76_RS15090: BWI76_RS15090 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 3,161,173 to 3,161,958
_RS15090
BWI76_RS15095: BWI76_RS15095 - isochorismatase, at 3,161,977 to 3,162,609
_RS15095
BWI76_RS15100: BWI76_RS15100 - phenazine biosynthesis protein PhzE, at 3,162,639 to 3,164,588
_RS15100
Position (kb)
3161
3162
3163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3160.981 kb on + strand, within BWI76_RS15085 at 3161.145 kb on - strand at 3161.190 kb on + strand at 3161.200 kb on - strand at 3161.270 kb on - strand, within BWI76_RS15090 at 3161.275 kb on + strand, within BWI76_RS15090 at 3161.314 kb on - strand, within BWI76_RS15090 at 3161.622 kb on + strand, within BWI76_RS15090 at 3161.719 kb on - strand, within BWI76_RS15090 at 3161.876 kb on + strand, within BWI76_RS15090 at 3161.884 kb on - strand at 3161.975 kb on - strand at 3161.991 kb on + strand at 3162.061 kb on - strand, within BWI76_RS15095 at 3162.120 kb on - strand at 3162.120 kb on - strand, within BWI76_RS15095 at 3162.229 kb on - strand, within BWI76_RS15095 at 3162.229 kb on - strand, within BWI76_RS15095 at 3162.545 kb on - strand, within BWI76_RS15095 at 3162.652 kb on - strand at 3162.720 kb on - strand at 3162.766 kb on + strand at 3163.103 kb on - strand, within BWI76_RS15100 at 3163.149 kb on + strand, within BWI76_RS15100 at 3163.200 kb on - strand at 3163.230 kb on - strand, within BWI76_RS15100 at 3163.297 kb on + strand, within BWI76_RS15100 at 3163.451 kb on + strand, within BWI76_RS15100 at 3163.491 kb on + strand, within BWI76_RS15100 at 3163.513 kb on + strand, within BWI76_RS15100 at 3163.572 kb on - strand, within BWI76_RS15100
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 0.1 remove 3,160,981 + BWI76_RS15085 0.83 +0.2 3,161,145 - -1.3 3,161,190 + +2.1 3,161,200 - +0.3 3,161,270 - BWI76_RS15090 0.12 -0.5 3,161,275 + BWI76_RS15090 0.13 -0.2 3,161,314 - BWI76_RS15090 0.18 +0.3 3,161,622 + BWI76_RS15090 0.57 +0.4 3,161,719 - BWI76_RS15090 0.69 +0.3 3,161,876 + BWI76_RS15090 0.89 -1.6 3,161,884 - -1.0 3,161,975 - -1.5 3,161,991 + +1.8 3,162,061 - BWI76_RS15095 0.13 -0.9 3,162,120 - +0.8 3,162,120 - BWI76_RS15095 0.23 -0.6 3,162,229 - BWI76_RS15095 0.40 -0.2 3,162,229 - BWI76_RS15095 0.40 +0.3 3,162,545 - BWI76_RS15095 0.90 -0.7 3,162,652 - -0.1 3,162,720 - -1.2 3,162,766 + +0.5 3,163,103 - BWI76_RS15100 0.24 +0.4 3,163,149 + BWI76_RS15100 0.26 -0.0 3,163,200 - -1.8 3,163,230 - BWI76_RS15100 0.30 -1.1 3,163,297 + BWI76_RS15100 0.34 -0.4 3,163,451 + BWI76_RS15100 0.42 -0.3 3,163,491 + BWI76_RS15100 0.44 +0.4 3,163,513 + BWI76_RS15100 0.45 -0.2 3,163,572 - BWI76_RS15100 0.48 +0.1
Or see this region's nucleotide sequence