Experiment: KLEB28 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10505 and BWI76_RS10510 overlap by 23 nucleotides BWI76_RS10510 and BWI76_RS10515 overlap by 4 nucleotides BWI76_RS10515 and BWI76_RS10520 are separated by 41 nucleotides
BWI76_RS10505: BWI76_RS10505 - putative guanine/xanthin permease, at 2,220,168 to 2,221,550
_RS10505
BWI76_RS10510: BWI76_RS10510 - OHCU decarboxylase, at 2,221,528 to 2,222,028
_RS10510
BWI76_RS10515: BWI76_RS10515 - 5-hydroxyisourate hydrolase, at 2,222,025 to 2,222,351
_RS10515
BWI76_RS10520: BWI76_RS10520 - guanine deaminase, at 2,222,393 to 2,223,712
_RS10520
Position (kb)
2221
2222
2223 Strain fitness (log2 ratio)
-1
0
1 at 2220.938 kb on + strand, within BWI76_RS10505 at 2221.105 kb on - strand, within BWI76_RS10505 at 2221.210 kb on - strand, within BWI76_RS10505 at 2221.796 kb on + strand, within BWI76_RS10510 at 2221.814 kb on - strand, within BWI76_RS10510 at 2221.970 kb on + strand, within BWI76_RS10510 at 2222.383 kb on + strand at 2222.483 kb on + strand at 2222.639 kb on + strand, within BWI76_RS10520 at 2222.647 kb on - strand, within BWI76_RS10520 at 2222.647 kb on - strand, within BWI76_RS10520 at 2222.711 kb on - strand, within BWI76_RS10520 at 2222.874 kb on + strand, within BWI76_RS10520 at 2222.973 kb on + strand, within BWI76_RS10520
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 0.1 remove 2,220,938 + BWI76_RS10505 0.56 +0.5 2,221,105 - BWI76_RS10505 0.68 -0.1 2,221,210 - BWI76_RS10505 0.75 +0.2 2,221,796 + BWI76_RS10510 0.53 +0.7 2,221,814 - BWI76_RS10510 0.57 +0.8 2,221,970 + BWI76_RS10510 0.88 -0.2 2,222,383 + -0.0 2,222,483 + +0.2 2,222,639 + BWI76_RS10520 0.19 -0.4 2,222,647 - BWI76_RS10520 0.19 -0.3 2,222,647 - BWI76_RS10520 0.19 -0.2 2,222,711 - BWI76_RS10520 0.24 +1.6 2,222,874 + BWI76_RS10520 0.36 -0.1 2,222,973 + BWI76_RS10520 0.44 +0.8
Or see this region's nucleotide sequence