Experiment: KLEB28 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06220 and BWI76_RS06225 overlap by 4 nucleotides BWI76_RS06225 and BWI76_RS06230 are separated by 294 nucleotides
BWI76_RS06220: BWI76_RS06220 - exonuclease subunit SbcC, at 1,303,115 to 1,306,249
_RS06220
BWI76_RS06225: BWI76_RS06225 - exonuclease sbcCD subunit D, at 1,306,246 to 1,307,448
_RS06225
BWI76_RS06230: BWI76_RS06230 - DNA-binding response regulator, at 1,307,743 to 1,308,432
_RS06230
Position (kb)
1306
1307
1308 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1305.804 kb on - strand, within BWI76_RS06220 at 1306.072 kb on - strand at 1306.072 kb on - strand at 1306.174 kb on + strand at 1306.296 kb on + strand at 1306.427 kb on - strand, within BWI76_RS06225 at 1306.854 kb on - strand, within BWI76_RS06225 at 1306.946 kb on - strand, within BWI76_RS06225 at 1307.040 kb on - strand, within BWI76_RS06225 at 1307.278 kb on + strand, within BWI76_RS06225 at 1307.278 kb on + strand, within BWI76_RS06225 at 1307.431 kb on + strand at 1307.432 kb on - strand at 1307.514 kb on - strand at 1307.514 kb on - strand at 1307.530 kb on + strand at 1307.540 kb on - strand at 1307.567 kb on + strand at 1307.575 kb on - strand at 1307.707 kb on + strand at 1307.707 kb on + strand at 1307.707 kb on + strand at 1307.855 kb on + strand, within BWI76_RS06230 at 1307.865 kb on + strand, within BWI76_RS06230 at 1307.933 kb on + strand, within BWI76_RS06230 at 1308.435 kb on + strand at 1308.444 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 0.1 remove 1,305,804 - BWI76_RS06220 0.86 -0.3 1,306,072 - -0.2 1,306,072 - -0.6 1,306,174 + -1.0 1,306,296 + +0.1 1,306,427 - BWI76_RS06225 0.15 -0.7 1,306,854 - BWI76_RS06225 0.51 +0.2 1,306,946 - BWI76_RS06225 0.58 -1.1 1,307,040 - BWI76_RS06225 0.66 -0.1 1,307,278 + BWI76_RS06225 0.86 -0.4 1,307,278 + BWI76_RS06225 0.86 -0.2 1,307,431 + -0.1 1,307,432 - +2.9 1,307,514 - -0.1 1,307,514 - +0.1 1,307,530 + -0.2 1,307,540 - +0.4 1,307,567 + -0.7 1,307,575 - -0.0 1,307,707 + +0.4 1,307,707 + -0.1 1,307,707 + +0.4 1,307,855 + BWI76_RS06230 0.16 +1.3 1,307,865 + BWI76_RS06230 0.18 -0.0 1,307,933 + BWI76_RS06230 0.28 +0.2 1,308,435 + -0.7 1,308,444 + +0.1
Or see this region's nucleotide sequence