Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10770 and BWI76_RS10775 overlap by 8 nucleotides BWI76_RS10775 and BWI76_RS10780 overlap by 1 nucleotides BWI76_RS10780 and BWI76_RS10785 are separated by 286 nucleotides BWI76_RS10785 and BWI76_RS10790 overlap by 4 nucleotides
BWI76_RS10770: BWI76_RS10770 - pyrimidine utilization protein C, at 2,273,743 to 2,274,135
_RS10770
BWI76_RS10775: BWI76_RS10775 - pyrimidine utilization protein B, at 2,274,128 to 2,274,838
_RS10775
BWI76_RS10780: BWI76_RS10780 - pyrimidine utilization protein A, at 2,274,838 to 2,275,929
_RS10780
BWI76_RS10785: BWI76_RS10785 - transcriptional regulator, at 2,276,216 to 2,276,854
_RS10785
BWI76_RS10790: BWI76_RS10790 - hypothetical protein, at 2,276,851 to 2,277,246
_RS10790
Position (kb)
2274
2275
2276 Strain fitness (log2 ratio)
-1
0
1 at 2273.851 kb on - strand, within BWI76_RS10770 at 2273.922 kb on + strand, within BWI76_RS10770 at 2274.148 kb on + strand at 2274.172 kb on + strand at 2274.274 kb on - strand, within BWI76_RS10775 at 2274.279 kb on + strand, within BWI76_RS10775 at 2274.392 kb on + strand, within BWI76_RS10775 at 2274.414 kb on + strand, within BWI76_RS10775 at 2274.529 kb on + strand, within BWI76_RS10775 at 2274.537 kb on - strand, within BWI76_RS10775 at 2274.735 kb on - strand, within BWI76_RS10775 at 2274.788 kb on + strand at 2274.796 kb on + strand at 2275.360 kb on + strand, within BWI76_RS10780 at 2275.368 kb on - strand, within BWI76_RS10780 at 2275.654 kb on - strand, within BWI76_RS10780 at 2275.784 kb on + strand, within BWI76_RS10780 at 2275.861 kb on + strand at 2275.864 kb on - strand at 2275.869 kb on - strand at 2275.984 kb on - strand at 2276.219 kb on + strand at 2276.219 kb on + strand at 2276.227 kb on - strand at 2276.227 kb on - strand at 2276.385 kb on + strand, within BWI76_RS10785 at 2276.393 kb on - strand, within BWI76_RS10785 at 2276.402 kb on - strand, within BWI76_RS10785 at 2276.482 kb on - strand, within BWI76_RS10785 at 2276.525 kb on - strand, within BWI76_RS10785 at 2276.525 kb on - strand, within BWI76_RS10785 at 2276.728 kb on + strand, within BWI76_RS10785 at 2276.728 kb on + strand, within BWI76_RS10785
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 2,273,851 - BWI76_RS10770 0.27 +0.0 2,273,922 + BWI76_RS10770 0.46 -0.4 2,274,148 + -1.1 2,274,172 + +0.7 2,274,274 - BWI76_RS10775 0.21 -0.6 2,274,279 + BWI76_RS10775 0.21 +0.1 2,274,392 + BWI76_RS10775 0.37 +0.1 2,274,414 + BWI76_RS10775 0.40 -0.1 2,274,529 + BWI76_RS10775 0.56 -0.1 2,274,537 - BWI76_RS10775 0.58 -0.2 2,274,735 - BWI76_RS10775 0.85 -0.1 2,274,788 + -0.1 2,274,796 + -0.0 2,275,360 + BWI76_RS10780 0.48 -0.7 2,275,368 - BWI76_RS10780 0.49 -1.3 2,275,654 - BWI76_RS10780 0.75 -0.1 2,275,784 + BWI76_RS10780 0.87 -0.3 2,275,861 + -0.2 2,275,864 - +0.2 2,275,869 - +0.0 2,275,984 - -0.6 2,276,219 + -0.3 2,276,219 + -0.6 2,276,227 - -0.6 2,276,227 - -0.0 2,276,385 + BWI76_RS10785 0.26 -0.7 2,276,393 - BWI76_RS10785 0.28 +0.2 2,276,402 - BWI76_RS10785 0.29 -0.0 2,276,482 - BWI76_RS10785 0.42 -0.2 2,276,525 - BWI76_RS10785 0.48 -1.2 2,276,525 - BWI76_RS10785 0.48 -0.4 2,276,728 + BWI76_RS10785 0.80 +0.7 2,276,728 + BWI76_RS10785 0.80 -0.1
Or see this region's nucleotide sequence