Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10420 and BWI76_RS10425 are separated by 43 nucleotides BWI76_RS10425 and BWI76_RS10430 are separated by 107 nucleotides BWI76_RS10430 and BWI76_RS10435 are separated by 44 nucleotides BWI76_RS10435 and BWI76_RS10440 are separated by 7 nucleotides
BWI76_RS10420: BWI76_RS10420 - monooxygenase, at 2,203,362 to 2,204,390
_RS10420
BWI76_RS10425: BWI76_RS10425 - AIG2 family protein, at 2,204,434 to 2,204,769
_RS10425
BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635
_RS10430
BWI76_RS10435: BWI76_RS10435 - suppressor for copper-sensitivity C, at 2,205,680 to 2,206,402
_RS10435
BWI76_RS10440: BWI76_RS10440 - protein-disulfide reductase, at 2,206,410 to 2,208,434
_RS10440
Position (kb)
2204
2205
2206 Strain fitness (log2 ratio)
-1
0
1 at 2203.880 kb on + strand, within BWI76_RS10420 at 2204.239 kb on - strand, within BWI76_RS10420 at 2204.329 kb on - strand at 2204.744 kb on - strand at 2205.247 kb on + strand, within BWI76_RS10430 at 2205.360 kb on + strand, within BWI76_RS10430 at 2205.445 kb on + strand, within BWI76_RS10430 at 2205.491 kb on - strand, within BWI76_RS10430 at 2205.589 kb on - strand at 2205.739 kb on - strand at 2205.802 kb on + strand, within BWI76_RS10435 at 2206.100 kb on + strand, within BWI76_RS10435 at 2206.108 kb on - strand, within BWI76_RS10435 at 2206.505 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 2,203,880 + BWI76_RS10420 0.50 +0.1 2,204,239 - BWI76_RS10420 0.85 -0.2 2,204,329 - -0.1 2,204,744 - -0.2 2,205,247 + BWI76_RS10430 0.49 -1.0 2,205,360 + BWI76_RS10430 0.64 -0.5 2,205,445 + BWI76_RS10430 0.75 -0.6 2,205,491 - BWI76_RS10430 0.81 -0.5 2,205,589 - -0.0 2,205,739 - -0.0 2,205,802 + BWI76_RS10435 0.17 +0.4 2,206,100 + BWI76_RS10435 0.58 -0.0 2,206,108 - BWI76_RS10435 0.59 -0.0 2,206,505 + -0.2
Or see this region's nucleotide sequence