Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01510 and BWI76_RS01515 are separated by 37 nucleotides BWI76_RS01515 and BWI76_RS01520 are separated by 3 nucleotides BWI76_RS01520 and BWI76_RS01525 are separated by 172 nucleotides BWI76_RS01525 and BWI76_RS01530 are separated by 318 nucleotides
BWI76_RS01510: BWI76_RS01510 - anaerobic C4-dicarboxylate transporter, at 308,526 to 309,866
_RS01510
BWI76_RS01515: BWI76_RS01515 - PTS family cellobiose transporter subunit IIA, at 309,904 to 310,221
_RS01515
BWI76_RS01520: BWI76_RS01520 - PTS sugar transporter subunit IIB, at 310,225 to 310,530
_RS01520
BWI76_RS01525: BWI76_RS01525 - sensor domain-containing diguanylate cyclase, at 310,703 to 312,235
_RS01525
BWI76_RS01530: BWI76_RS01530 - 2-iminoacetate synthase ThiH, at 312,554 to 313,687
_RS01530
Position (kb) 310
311
312
313 Strain fitness (log2 ratio) -2
-1
0
1
2 at 309.747 kb on + strand at 309.976 kb on + strand, within BWI76_RS01515 at 309.981 kb on + strand, within BWI76_RS01515 at 310.030 kb on + strand, within BWI76_RS01515 at 310.030 kb on + strand, within BWI76_RS01515 at 310.038 kb on - strand, within BWI76_RS01515 at 310.178 kb on + strand, within BWI76_RS01515 at 310.237 kb on + strand at 310.325 kb on + strand, within BWI76_RS01520 at 310.325 kb on + strand, within BWI76_RS01520 at 310.325 kb on + strand, within BWI76_RS01520 at 310.325 kb on + strand, within BWI76_RS01520 at 310.333 kb on - strand, within BWI76_RS01520 at 310.359 kb on + strand, within BWI76_RS01520 at 310.518 kb on - strand at 310.548 kb on - strand at 310.616 kb on - strand at 310.695 kb on - strand at 310.810 kb on - strand at 311.006 kb on - strand, within BWI76_RS01525 at 311.006 kb on - strand, within BWI76_RS01525 at 311.170 kb on - strand, within BWI76_RS01525 at 311.205 kb on - strand, within BWI76_RS01525 at 311.293 kb on - strand, within BWI76_RS01525 at 311.394 kb on - strand, within BWI76_RS01525 at 311.450 kb on + strand, within BWI76_RS01525 at 311.484 kb on + strand, within BWI76_RS01525 at 311.527 kb on - strand, within BWI76_RS01525 at 311.588 kb on + strand, within BWI76_RS01525 at 311.657 kb on - strand, within BWI76_RS01525 at 311.679 kb on - strand, within BWI76_RS01525 at 311.712 kb on + strand, within BWI76_RS01525 at 311.720 kb on - strand, within BWI76_RS01525 at 311.722 kb on + strand, within BWI76_RS01525 at 311.742 kb on - strand, within BWI76_RS01525 at 311.763 kb on + strand, within BWI76_RS01525 at 311.781 kb on - strand, within BWI76_RS01525 at 311.840 kb on + strand, within BWI76_RS01525 at 312.239 kb on - strand at 312.259 kb on + strand at 312.265 kb on - strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.282 kb on + strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.290 kb on - strand at 312.337 kb on - strand at 312.392 kb on + strand at 312.399 kb on + strand at 312.406 kb on - strand at 312.416 kb on - strand at 312.435 kb on + strand at 312.443 kb on - strand at 312.443 kb on - strand at 312.452 kb on + strand at 312.460 kb on - strand at 312.462 kb on + strand at 312.488 kb on + strand at 312.562 kb on + strand at 312.570 kb on - strand at 312.570 kb on - strand at 312.570 kb on - strand at 312.577 kb on + strand at 312.587 kb on + strand at 312.596 kb on - strand at 312.643 kb on + strand at 312.666 kb on + strand at 312.674 kb on - strand, within BWI76_RS01530 at 312.674 kb on - strand, within BWI76_RS01530 at 312.941 kb on + strand, within BWI76_RS01530 at 313.038 kb on + strand, within BWI76_RS01530
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 309,747 + +0.5 309,976 + BWI76_RS01515 0.23 +0.1 309,981 + BWI76_RS01515 0.24 +0.3 310,030 + BWI76_RS01515 0.40 -1.2 310,030 + BWI76_RS01515 0.40 +0.5 310,038 - BWI76_RS01515 0.42 +0.1 310,178 + BWI76_RS01515 0.86 +0.5 310,237 + -1.1 310,325 + BWI76_RS01520 0.33 +0.0 310,325 + BWI76_RS01520 0.33 +0.0 310,325 + BWI76_RS01520 0.33 +0.3 310,325 + BWI76_RS01520 0.33 +1.2 310,333 - BWI76_RS01520 0.35 -0.2 310,359 + BWI76_RS01520 0.44 -0.1 310,518 - +0.6 310,548 - -0.2 310,616 - +0.0 310,695 - +0.3 310,810 - +0.8 311,006 - BWI76_RS01525 0.20 +0.6 311,006 - BWI76_RS01525 0.20 -0.0 311,170 - BWI76_RS01525 0.30 -0.8 311,205 - BWI76_RS01525 0.33 +0.8 311,293 - BWI76_RS01525 0.38 +0.6 311,394 - BWI76_RS01525 0.45 +0.3 311,450 + BWI76_RS01525 0.49 +0.0 311,484 + BWI76_RS01525 0.51 +0.1 311,527 - BWI76_RS01525 0.54 +0.5 311,588 + BWI76_RS01525 0.58 +0.1 311,657 - BWI76_RS01525 0.62 +1.1 311,679 - BWI76_RS01525 0.64 +0.4 311,712 + BWI76_RS01525 0.66 +0.9 311,720 - BWI76_RS01525 0.66 -0.0 311,722 + BWI76_RS01525 0.66 +0.4 311,742 - BWI76_RS01525 0.68 +0.0 311,763 + BWI76_RS01525 0.69 +2.1 311,781 - BWI76_RS01525 0.70 -0.1 311,840 + BWI76_RS01525 0.74 +0.4 312,239 - +0.3 312,259 + +1.1 312,265 - +1.1 312,282 + -0.3 312,282 + +0.2 312,282 + -0.0 312,282 + +0.3 312,282 + -0.7 312,282 + +0.1 312,282 + +0.8 312,282 + +0.6 312,282 + +0.1 312,290 - +0.3 312,290 - +0.1 312,290 - +0.2 312,290 - +0.6 312,290 - +1.1 312,290 - +0.4 312,290 - -0.2 312,337 - +0.3 312,392 + -1.0 312,399 + +0.6 312,406 - +0.4 312,416 - +0.5 312,435 + +0.2 312,443 - +0.3 312,443 - +0.5 312,452 + +0.8 312,460 - -2.0 312,462 + +0.0 312,488 + -0.3 312,562 + +0.9 312,570 - +0.5 312,570 - +1.0 312,570 - +1.4 312,577 + +0.5 312,587 + -0.5 312,596 - +0.8 312,643 + +0.0 312,666 + +0.5 312,674 - BWI76_RS01530 0.11 +0.2 312,674 - BWI76_RS01530 0.11 -0.0 312,941 + BWI76_RS01530 0.34 +0.8 313,038 + BWI76_RS01530 0.43 +1.0
Or see this region's nucleotide sequence