Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17865 and BWI76_RS17870 are separated by 3 nucleotides BWI76_RS17870 and BWI76_RS17875 are separated by 162 nucleotides BWI76_RS17875 and BWI76_RS17880 are separated by 131 nucleotides
BWI76_RS17865: BWI76_RS17865 - aminodeoxychorismate synthase subunit I, at 3,714,375 to 3,715,730
_RS17865
BWI76_RS17870: BWI76_RS17870 - coenzyme A pyrophosphatase, at 3,715,734 to 3,716,312
_RS17870
BWI76_RS17875: BWI76_RS17875 - L-serine ammonia-lyase, at 3,716,475 to 3,717,839
_RS17875
BWI76_RS17880: BWI76_RS17880 - c-di-GMP phosphodiesterase, at 3,717,971 to 3,719,536
_RS17880
Position (kb) 3716
3717
3718 Strain fitness (log2 ratio) -1
0
1 at 3715.940 kb on + strand, within BWI76_RS17870 at 3715.964 kb on - strand, within BWI76_RS17870 at 3716.079 kb on - strand, within BWI76_RS17870 at 3716.091 kb on - strand, within BWI76_RS17870 at 3716.219 kb on + strand, within BWI76_RS17870 at 3716.236 kb on - strand, within BWI76_RS17870 at 3716.320 kb on - strand at 3717.066 kb on + strand, within BWI76_RS17875 at 3717.504 kb on + strand, within BWI76_RS17875 at 3717.569 kb on - strand, within BWI76_RS17875 at 3717.635 kb on + strand, within BWI76_RS17875 at 3717.723 kb on + strand at 3717.799 kb on - strand at 3717.915 kb on - strand at 3718.082 kb on - strand at 3718.178 kb on + strand, within BWI76_RS17880 at 3718.401 kb on + strand, within BWI76_RS17880 at 3718.667 kb on - strand, within BWI76_RS17880
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 3,715,940 + BWI76_RS17870 0.36 -0.2 3,715,964 - BWI76_RS17870 0.40 +0.2 3,716,079 - BWI76_RS17870 0.60 -0.3 3,716,091 - BWI76_RS17870 0.62 +0.2 3,716,219 + BWI76_RS17870 0.84 +0.2 3,716,236 - BWI76_RS17870 0.87 +0.2 3,716,320 - +0.4 3,717,066 + BWI76_RS17875 0.43 -0.2 3,717,504 + BWI76_RS17875 0.75 -0.7 3,717,569 - BWI76_RS17875 0.80 -0.1 3,717,635 + BWI76_RS17875 0.85 -0.9 3,717,723 + +0.0 3,717,799 - +0.9 3,717,915 - -0.5 3,718,082 - +0.1 3,718,178 + BWI76_RS17880 0.13 +0.1 3,718,401 + BWI76_RS17880 0.27 +0.8 3,718,667 - BWI76_RS17880 0.44 -0.3
Or see this region's nucleotide sequence