Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10710 and BWI76_RS10715 are separated by 171 nucleotides BWI76_RS10715 and BWI76_RS10720 overlap by 11 nucleotides BWI76_RS10720 and BWI76_RS10725 are separated by 516 nucleotides BWI76_RS10725 and BWI76_RS10730 are separated by 139 nucleotides
BWI76_RS10710: BWI76_RS10710 - Glutamate--putrescine ligase (EC 6.3.1.11) (from data) , at 2,263,423 to 2,264,841
_RS10710
BWI76_RS10715: BWI76_RS10715 - hypothetical protein, at 2,265,013 to 2,265,201
_RS10715
BWI76_RS10720: BWI76_RS10720 - putrescine importer, at 2,265,191 to 2,266,582
_RS10720
BWI76_RS10725: BWI76_RS10725 - hypothetical protein, at 2,267,099 to 2,267,323
_RS10725
BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710
_RS10730
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2264.266 kb on + strand, within BWI76_RS10710 at 2264.645 kb on + strand, within BWI76_RS10710 at 2264.650 kb on + strand, within BWI76_RS10710 at 2264.949 kb on + strand at 2264.949 kb on + strand at 2264.990 kb on - strand at 2264.994 kb on + strand at 2265.219 kb on + strand at 2265.219 kb on + strand at 2265.260 kb on - strand at 2265.446 kb on + strand, within BWI76_RS10720 at 2265.522 kb on - strand, within BWI76_RS10720 at 2265.582 kb on - strand, within BWI76_RS10720 at 2265.602 kb on - strand, within BWI76_RS10720 at 2266.112 kb on - strand, within BWI76_RS10720 at 2266.492 kb on - strand at 2266.506 kb on - strand at 2266.551 kb on - strand at 2266.567 kb on + strand at 2266.567 kb on + strand at 2266.570 kb on - strand at 2266.598 kb on + strand at 2266.739 kb on - strand at 2266.776 kb on + strand at 2266.776 kb on + strand at 2267.058 kb on + strand at 2267.064 kb on + strand at 2267.064 kb on + strand at 2267.066 kb on - strand at 2267.148 kb on - strand, within BWI76_RS10725 at 2267.264 kb on - strand, within BWI76_RS10725 at 2267.502 kb on + strand at 2267.510 kb on - strand at 2267.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 2,264,266 + BWI76_RS10710 0.59 -0.2 2,264,645 + BWI76_RS10710 0.86 +0.2 2,264,650 + BWI76_RS10710 0.86 +0.2 2,264,949 + +0.2 2,264,949 + +0.0 2,264,990 - +0.3 2,264,994 + +0.5 2,265,219 + -1.3 2,265,219 + +0.2 2,265,260 - +0.6 2,265,446 + BWI76_RS10720 0.18 -0.3 2,265,522 - BWI76_RS10720 0.24 -0.8 2,265,582 - BWI76_RS10720 0.28 -0.3 2,265,602 - BWI76_RS10720 0.30 -0.3 2,266,112 - BWI76_RS10720 0.66 -0.7 2,266,492 - +0.2 2,266,506 - +0.8 2,266,551 - -0.1 2,266,567 + +0.1 2,266,567 + -0.1 2,266,570 - -0.3 2,266,598 + +0.2 2,266,739 - -2.1 2,266,776 + +0.3 2,266,776 + +0.7 2,267,058 + +1.8 2,267,064 + -0.6 2,267,064 + +1.1 2,267,066 - -0.8 2,267,148 - BWI76_RS10725 0.22 -0.5 2,267,264 - BWI76_RS10725 0.73 +0.2 2,267,502 + +0.3 2,267,510 - -0.1 2,267,535 - -0.6
Or see this region's nucleotide sequence