Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10720

Experiment: KLEB28 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10710 and BWI76_RS10715 are separated by 171 nucleotidesBWI76_RS10715 and BWI76_RS10720 overlap by 11 nucleotidesBWI76_RS10720 and BWI76_RS10725 are separated by 516 nucleotidesBWI76_RS10725 and BWI76_RS10730 are separated by 139 nucleotides BWI76_RS10710: BWI76_RS10710 - Glutamate--putrescine ligase (EC 6.3.1.11) (from data), at 2,263,423 to 2,264,841 _RS10710 BWI76_RS10715: BWI76_RS10715 - hypothetical protein, at 2,265,013 to 2,265,201 _RS10715 BWI76_RS10720: BWI76_RS10720 - putrescine importer, at 2,265,191 to 2,266,582 _RS10720 BWI76_RS10725: BWI76_RS10725 - hypothetical protein, at 2,267,099 to 2,267,323 _RS10725 BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710 _RS10730 Position (kb) 2265 2266 2267Strain fitness (log2 ratio) -2 -1 0 1 2at 2264.266 kb on + strand, within BWI76_RS10710at 2264.645 kb on + strand, within BWI76_RS10710at 2264.650 kb on + strand, within BWI76_RS10710at 2264.949 kb on + strandat 2264.949 kb on + strandat 2264.990 kb on - strandat 2264.994 kb on + strandat 2265.219 kb on + strandat 2265.219 kb on + strandat 2265.260 kb on - strandat 2265.446 kb on + strand, within BWI76_RS10720at 2265.522 kb on - strand, within BWI76_RS10720at 2265.582 kb on - strand, within BWI76_RS10720at 2265.602 kb on - strand, within BWI76_RS10720at 2266.112 kb on - strand, within BWI76_RS10720at 2266.492 kb on - strandat 2266.506 kb on - strandat 2266.551 kb on - strandat 2266.567 kb on + strandat 2266.567 kb on + strandat 2266.570 kb on - strandat 2266.598 kb on + strandat 2266.739 kb on - strandat 2266.776 kb on + strandat 2266.776 kb on + strandat 2267.058 kb on + strandat 2267.064 kb on + strandat 2267.064 kb on + strandat 2267.066 kb on - strandat 2267.148 kb on - strand, within BWI76_RS10725at 2267.264 kb on - strand, within BWI76_RS10725at 2267.502 kb on + strandat 2267.510 kb on - strandat 2267.535 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB28 MOI 1
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2,264,266 + BWI76_RS10710 0.59 -0.2
2,264,645 + BWI76_RS10710 0.86 +0.2
2,264,650 + BWI76_RS10710 0.86 +0.2
2,264,949 + +0.2
2,264,949 + +0.0
2,264,990 - +0.3
2,264,994 + +0.5
2,265,219 + -1.3
2,265,219 + +0.2
2,265,260 - +0.6
2,265,446 + BWI76_RS10720 0.18 -0.3
2,265,522 - BWI76_RS10720 0.24 -0.8
2,265,582 - BWI76_RS10720 0.28 -0.3
2,265,602 - BWI76_RS10720 0.30 -0.3
2,266,112 - BWI76_RS10720 0.66 -0.7
2,266,492 - +0.2
2,266,506 - +0.8
2,266,551 - -0.1
2,266,567 + +0.1
2,266,567 + -0.1
2,266,570 - -0.3
2,266,598 + +0.2
2,266,739 - -2.1
2,266,776 + +0.3
2,266,776 + +0.7
2,267,058 + +1.8
2,267,064 + -0.6
2,267,064 + +1.1
2,267,066 - -0.8
2,267,148 - BWI76_RS10725 0.22 -0.5
2,267,264 - BWI76_RS10725 0.73 +0.2
2,267,502 + +0.3
2,267,510 - -0.1
2,267,535 - -0.6

Or see this region's nucleotide sequence