Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10505

Experiment: KLEB28 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10500 and BWI76_RS10505 are separated by 346 nucleotidesBWI76_RS10505 and BWI76_RS10510 overlap by 23 nucleotidesBWI76_RS10510 and BWI76_RS10515 overlap by 4 nucleotidesBWI76_RS10515 and BWI76_RS10520 are separated by 41 nucleotides BWI76_RS10500: BWI76_RS10500 - flavoprotein monooxygenase, at 2,218,667 to 2,219,821 _RS10500 BWI76_RS10505: BWI76_RS10505 - putative guanine/xanthin permease, at 2,220,168 to 2,221,550 _RS10505 BWI76_RS10510: BWI76_RS10510 - OHCU decarboxylase, at 2,221,528 to 2,222,028 _RS10510 BWI76_RS10515: BWI76_RS10515 - 5-hydroxyisourate hydrolase, at 2,222,025 to 2,222,351 _RS10515 BWI76_RS10520: BWI76_RS10520 - guanine deaminase, at 2,222,393 to 2,223,712 _RS10520 Position (kb) 2220 2221 2222Strain fitness (log2 ratio) -1 0 1at 2219.578 kb on + strand, within BWI76_RS10500at 2219.656 kb on - strand, within BWI76_RS10500at 2220.354 kb on - strand, within BWI76_RS10505at 2220.938 kb on + strand, within BWI76_RS10505at 2221.105 kb on - strand, within BWI76_RS10505at 2221.210 kb on - strand, within BWI76_RS10505at 2221.796 kb on + strand, within BWI76_RS10510at 2221.814 kb on - strand, within BWI76_RS10510at 2221.970 kb on + strand, within BWI76_RS10510at 2222.383 kb on + strandat 2222.483 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB28 MOI 1
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2,219,578 + BWI76_RS10500 0.79 -0.4
2,219,656 - BWI76_RS10500 0.86 +0.5
2,220,354 - BWI76_RS10505 0.13 -0.4
2,220,938 + BWI76_RS10505 0.56 -0.1
2,221,105 - BWI76_RS10505 0.68 -0.3
2,221,210 - BWI76_RS10505 0.75 -1.1
2,221,796 + BWI76_RS10510 0.53 +0.0
2,221,814 - BWI76_RS10510 0.57 -0.0
2,221,970 + BWI76_RS10510 0.88 +0.1
2,222,383 + -0.1
2,222,483 + -0.1

Or see this region's nucleotide sequence