Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06170 and BWI76_RS06175 are separated by 116 nucleotides BWI76_RS06175 and BWI76_RS06180 overlap by 4 nucleotides BWI76_RS06180 and BWI76_RS06185 are separated by 334 nucleotides BWI76_RS06185 and BWI76_RS06190 are separated by 71 nucleotides
BWI76_RS06170: BWI76_RS06170 - pyrroline-5-carboxylate reductase, at 1,295,963 to 1,296,772
_RS06170
BWI76_RS06175: BWI76_RS06175 - DUF188 domain-containing protein, at 1,296,889 to 1,297,347
_RS06175
BWI76_RS06180: BWI76_RS06180 - hypothetical protein, at 1,297,344 to 1,298,042
_RS06180
BWI76_RS06185: BWI76_RS06185 - shikimate kinase II, at 1,298,377 to 1,298,904
_RS06185
BWI76_RS06190: BWI76_RS06190 - hypothetical protein, at 1,298,976 to 1,299,167
_RS06190
Position (kb)
1297
1298
1299 Strain fitness (log2 ratio)
-1
0
1
2 at 1296.958 kb on - strand, within BWI76_RS06175 at 1297.039 kb on + strand, within BWI76_RS06175 at 1297.173 kb on + strand, within BWI76_RS06175 at 1297.405 kb on + strand at 1297.546 kb on - strand, within BWI76_RS06180 at 1297.580 kb on - strand, within BWI76_RS06180 at 1297.853 kb on + strand, within BWI76_RS06180 at 1298.038 kb on - strand at 1298.039 kb on + strand at 1298.052 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.125 kb on - strand at 1298.321 kb on - strand at 1298.359 kb on + strand at 1298.367 kb on - strand at 1298.367 kb on - strand at 1298.456 kb on + strand, within BWI76_RS06185 at 1298.464 kb on - strand, within BWI76_RS06185 at 1298.500 kb on - strand, within BWI76_RS06185 at 1298.615 kb on + strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.623 kb on - strand, within BWI76_RS06185 at 1298.645 kb on - strand, within BWI76_RS06185 at 1298.672 kb on + strand, within BWI76_RS06185 at 1298.705 kb on - strand, within BWI76_RS06185 at 1298.762 kb on + strand, within BWI76_RS06185 at 1298.805 kb on - strand, within BWI76_RS06185 at 1298.898 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 1,296,958 - BWI76_RS06175 0.15 -0.5 1,297,039 + BWI76_RS06175 0.33 -0.2 1,297,173 + BWI76_RS06175 0.62 -0.0 1,297,405 + +0.4 1,297,546 - BWI76_RS06180 0.29 -1.3 1,297,580 - BWI76_RS06180 0.34 -0.1 1,297,853 + BWI76_RS06180 0.73 -0.3 1,298,038 - -0.0 1,298,039 + +0.4 1,298,052 - +0.9 1,298,125 - +0.5 1,298,125 - -0.1 1,298,125 - -0.3 1,298,321 - -0.5 1,298,359 + +0.2 1,298,367 - -0.6 1,298,367 - +2.6 1,298,456 + BWI76_RS06185 0.15 +0.5 1,298,464 - BWI76_RS06185 0.16 -0.1 1,298,500 - BWI76_RS06185 0.23 -1.0 1,298,615 + BWI76_RS06185 0.45 +0.5 1,298,623 - BWI76_RS06185 0.47 -0.1 1,298,623 - BWI76_RS06185 0.47 +0.3 1,298,623 - BWI76_RS06185 0.47 +2.5 1,298,623 - BWI76_RS06185 0.47 +0.1 1,298,645 - BWI76_RS06185 0.51 -1.5 1,298,672 + BWI76_RS06185 0.56 -0.5 1,298,705 - BWI76_RS06185 0.62 -0.1 1,298,762 + BWI76_RS06185 0.73 -0.1 1,298,805 - BWI76_RS06185 0.81 +0.1 1,298,898 - -0.3
Or see this region's nucleotide sequence