Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03175 and BWI76_RS03180 are separated by 550 nucleotides BWI76_RS03180 and BWI76_RS03185 overlap by 4 nucleotides BWI76_RS03185 and BWI76_RS03190 overlap by 4 nucleotides BWI76_RS03190 and BWI76_RS03195 overlap by 4 nucleotides
BWI76_RS03175: BWI76_RS03175 - hypothetical protein, at 655,058 to 655,795
_RS03175
BWI76_RS03180: BWI76_RS03180 - phage transcriptional regulator, AlpA, at 656,346 to 656,612
_RS03180
BWI76_RS03185: BWI76_RS03185 - CI repressor, at 656,609 to 657,166
_RS03185
BWI76_RS03190: BWI76_RS03190 - hypothetical protein, at 657,163 to 657,390
_RS03190
BWI76_RS03195: BWI76_RS03195 - P4 phage protein, at 657,387 to 657,707
_RS03195
Position (kb) 656
657 Strain fitness (log2 ratio) -2
-1
0
1 at 655.377 kb on - strand, within BWI76_RS03175 at 655.421 kb on - strand, within BWI76_RS03175 at 655.481 kb on - strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.733 kb on + strand at 656.051 kb on + strand at 656.059 kb on - strand at 656.059 kb on - strand at 656.292 kb on + strand at 656.402 kb on + strand, within BWI76_RS03180 at 656.402 kb on + strand, within BWI76_RS03180 at 656.410 kb on - strand, within BWI76_RS03180 at 656.784 kb on - strand, within BWI76_RS03185 at 656.802 kb on + strand, within BWI76_RS03185 at 656.868 kb on - strand, within BWI76_RS03185 at 656.960 kb on - strand, within BWI76_RS03185 at 656.994 kb on - strand, within BWI76_RS03185 at 657.258 kb on + strand, within BWI76_RS03190 at 657.260 kb on + strand, within BWI76_RS03190 at 657.330 kb on + strand, within BWI76_RS03190 at 657.416 kb on - strand at 657.435 kb on + strand, within BWI76_RS03195 at 657.453 kb on - strand, within BWI76_RS03195 at 657.476 kb on - strand, within BWI76_RS03195
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 655,377 - BWI76_RS03175 0.43 -0.1 655,421 - BWI76_RS03175 0.49 +0.7 655,481 - BWI76_RS03175 0.57 +0.3 655,714 + BWI76_RS03175 0.89 -0.0 655,714 + BWI76_RS03175 0.89 +0.5 655,733 + +0.1 656,051 + -0.9 656,059 - -0.0 656,059 - -0.1 656,292 + +0.8 656,402 + BWI76_RS03180 0.21 -2.0 656,402 + BWI76_RS03180 0.21 +0.2 656,410 - BWI76_RS03180 0.24 -1.5 656,784 - BWI76_RS03185 0.31 -0.5 656,802 + BWI76_RS03185 0.35 +0.4 656,868 - BWI76_RS03185 0.46 -0.0 656,960 - BWI76_RS03185 0.63 -1.3 656,994 - BWI76_RS03185 0.69 -1.8 657,258 + BWI76_RS03190 0.42 -1.0 657,260 + BWI76_RS03190 0.43 -0.0 657,330 + BWI76_RS03190 0.73 -0.9 657,416 - +0.1 657,435 + BWI76_RS03195 0.15 +0.2 657,453 - BWI76_RS03195 0.21 -0.2 657,476 - BWI76_RS03195 0.28 -0.4
Or see this region's nucleotide sequence