Experiment: KLEB28 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00580 and BWI76_RS00585 are separated by 17 nucleotides BWI76_RS00585 and BWI76_RS00590 are separated by 15 nucleotides BWI76_RS00590 and BWI76_RS00595 overlap by 4 nucleotides
BWI76_RS00580: BWI76_RS00580 - ribokinase, at 115,688 to 116,899
_RS00580
BWI76_RS00585: BWI76_RS00585 - dihydrodipicolinate synthase family protein, at 116,917 to 117,840
_RS00585
BWI76_RS00590: BWI76_RS00590 - hypothetical protein, at 117,856 to 118,347
_RS00590
BWI76_RS00595: BWI76_RS00595 - MFS transporter, at 118,344 to 119,621
_RS00595
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-2
-1
0
1 at 116.872 kb on + strand at 116.879 kb on + strand at 116.884 kb on + strand at 116.884 kb on + strand at 116.884 kb on + strand at 116.884 kb on + strand at 116.887 kb on - strand at 116.892 kb on - strand at 116.892 kb on - strand at 116.892 kb on - strand at 116.892 kb on - strand at 116.892 kb on - strand at 116.892 kb on - strand at 116.893 kb on + strand at 116.893 kb on + strand at 116.893 kb on + strand at 116.894 kb on - strand at 116.894 kb on - strand at 116.901 kb on - strand at 116.950 kb on - strand at 117.034 kb on - strand, within BWI76_RS00585 at 117.034 kb on - strand, within BWI76_RS00585 at 117.072 kb on - strand, within BWI76_RS00585 at 117.077 kb on + strand, within BWI76_RS00585 at 117.112 kb on - strand, within BWI76_RS00585 at 117.115 kb on - strand, within BWI76_RS00585 at 117.117 kb on - strand, within BWI76_RS00585 at 117.215 kb on - strand, within BWI76_RS00585 at 117.250 kb on - strand, within BWI76_RS00585 at 117.285 kb on + strand, within BWI76_RS00585 at 117.331 kb on + strand at 117.431 kb on + strand, within BWI76_RS00585 at 117.444 kb on - strand, within BWI76_RS00585 at 117.451 kb on - strand, within BWI76_RS00585 at 117.488 kb on - strand, within BWI76_RS00585 at 117.542 kb on + strand, within BWI76_RS00585 at 117.564 kb on + strand, within BWI76_RS00585 at 117.577 kb on + strand, within BWI76_RS00585 at 117.795 kb on + strand at 117.801 kb on + strand at 117.829 kb on + strand at 117.859 kb on + strand at 117.891 kb on + strand at 117.899 kb on - strand at 118.113 kb on - strand, within BWI76_RS00590 at 118.290 kb on - strand, within BWI76_RS00590 at 118.448 kb on - strand at 118.450 kb on + strand at 118.458 kb on - strand at 118.524 kb on + strand, within BWI76_RS00595 at 118.623 kb on + strand, within BWI76_RS00595 at 118.631 kb on + strand, within BWI76_RS00595 at 118.644 kb on - strand, within BWI76_RS00595 at 118.757 kb on - strand, within BWI76_RS00595 at 118.914 kb on + strand, within BWI76_RS00595 at 118.951 kb on + strand, within BWI76_RS00595 at 118.970 kb on - strand, within BWI76_RS00595 at 118.999 kb on - strand, within BWI76_RS00595 at 119.012 kb on + strand, within BWI76_RS00595 at 119.120 kb on + strand, within BWI76_RS00595 at 119.151 kb on - strand, within BWI76_RS00595 at 119.242 kb on - strand, within BWI76_RS00595 at 119.259 kb on - strand, within BWI76_RS00595 at 119.274 kb on + strand, within BWI76_RS00595
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB28 MOI 1 remove 116,872 + +0.6 116,879 + -1.6 116,884 + -1.2 116,884 + -1.2 116,884 + -0.1 116,884 + -0.4 116,887 - +0.3 116,892 - -0.5 116,892 - -1.0 116,892 - -0.2 116,892 - +0.3 116,892 - -0.8 116,892 - -0.0 116,893 + -0.4 116,893 + -0.5 116,893 + -2.1 116,894 - -0.4 116,894 - +0.3 116,901 - -0.1 116,950 - -0.7 117,034 - BWI76_RS00585 0.13 -0.7 117,034 - BWI76_RS00585 0.13 -0.4 117,072 - BWI76_RS00585 0.17 -0.3 117,077 + BWI76_RS00585 0.17 -0.0 117,112 - BWI76_RS00585 0.21 +0.6 117,115 - BWI76_RS00585 0.21 +0.1 117,117 - BWI76_RS00585 0.22 +0.3 117,215 - BWI76_RS00585 0.32 -0.2 117,250 - BWI76_RS00585 0.36 -1.8 117,285 + BWI76_RS00585 0.40 -0.3 117,331 + +0.4 117,431 + BWI76_RS00585 0.56 +0.4 117,444 - BWI76_RS00585 0.57 -0.1 117,451 - BWI76_RS00585 0.58 +0.5 117,488 - BWI76_RS00585 0.62 +0.5 117,542 + BWI76_RS00585 0.68 +0.6 117,564 + BWI76_RS00585 0.70 +1.5 117,577 + BWI76_RS00585 0.71 -0.3 117,795 + -0.9 117,801 + -0.6 117,829 + +0.1 117,859 + -0.1 117,891 + +0.1 117,899 - -0.2 118,113 - BWI76_RS00590 0.52 -0.6 118,290 - BWI76_RS00590 0.88 -0.4 118,448 - -0.0 118,450 + +0.2 118,458 - -1.0 118,524 + BWI76_RS00595 0.14 +0.0 118,623 + BWI76_RS00595 0.22 -0.4 118,631 + BWI76_RS00595 0.22 -2.0 118,644 - BWI76_RS00595 0.23 +0.0 118,757 - BWI76_RS00595 0.32 +1.7 118,914 + BWI76_RS00595 0.45 -0.0 118,951 + BWI76_RS00595 0.47 +0.1 118,970 - BWI76_RS00595 0.49 -0.4 118,999 - BWI76_RS00595 0.51 -0.1 119,012 + BWI76_RS00595 0.52 +0.2 119,120 + BWI76_RS00595 0.61 +0.6 119,151 - BWI76_RS00595 0.63 +0.6 119,242 - BWI76_RS00595 0.70 -0.3 119,259 - BWI76_RS00595 0.72 +0.6 119,274 + BWI76_RS00595 0.73 +1.1
Or see this region's nucleotide sequence