Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23205

Experiment: KLEB25 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23200 and BWI76_RS23205 are separated by 91 nucleotidesBWI76_RS23205 and BWI76_RS23210 are separated by 148 nucleotides BWI76_RS23200: BWI76_RS23200 - propionate kinase, at 4,832,182 to 4,833,396 _RS23200 BWI76_RS23205: BWI76_RS23205 - propanediol utilization protein PduX, at 4,833,488 to 4,834,354 _RS23205 BWI76_RS23210: BWI76_RS23210 - threonine-phosphate decarboxylase, at 4,834,503 to 4,835,582 _RS23210 Position (kb) 4833 4834 4835Strain fitness (log2 ratio) -2 -1 0 1at 4832.711 kb on + strand, within BWI76_RS23200at 4832.898 kb on + strand, within BWI76_RS23200at 4832.933 kb on + strand, within BWI76_RS23200at 4832.967 kb on + strand, within BWI76_RS23200at 4833.292 kb on - strandat 4833.292 kb on - strandat 4833.309 kb on + strandat 4833.363 kb on + strandat 4833.580 kb on + strand, within BWI76_RS23205at 4833.670 kb on + strand, within BWI76_RS23205at 4833.745 kb on + strand, within BWI76_RS23205at 4833.863 kb on - strand, within BWI76_RS23205at 4833.887 kb on + strand, within BWI76_RS23205at 4834.041 kb on - strand, within BWI76_RS23205at 4834.336 kb on - strandat 4834.338 kb on + strandat 4834.340 kb on + strandat 4834.346 kb on - strandat 4834.348 kb on + strandat 4834.348 kb on + strandat 4834.536 kb on - strandat 4834.599 kb on - strandat 4834.697 kb on + strand, within BWI76_RS23210at 4834.701 kb on + strand, within BWI76_RS23210at 4835.216 kb on + strand, within BWI76_RS23210

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB25 MOI 0.1
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4,832,711 + BWI76_RS23200 0.44 +0.3
4,832,898 + BWI76_RS23200 0.59 +0.0
4,832,933 + BWI76_RS23200 0.62 +0.2
4,832,967 + BWI76_RS23200 0.65 +0.4
4,833,292 - -0.3
4,833,292 - +0.3
4,833,309 + +0.1
4,833,363 + +0.9
4,833,580 + BWI76_RS23205 0.11 -0.7
4,833,670 + BWI76_RS23205 0.21 -0.2
4,833,745 + BWI76_RS23205 0.30 +0.2
4,833,863 - BWI76_RS23205 0.43 -1.6
4,833,887 + BWI76_RS23205 0.46 -0.5
4,834,041 - BWI76_RS23205 0.64 -1.9
4,834,336 - -0.1
4,834,338 + +0.1
4,834,340 + -0.6
4,834,346 - -0.3
4,834,348 + -0.2
4,834,348 + +0.7
4,834,536 - -0.4
4,834,599 - -0.2
4,834,697 + BWI76_RS23210 0.18 -0.5
4,834,701 + BWI76_RS23210 0.18 -0.1
4,835,216 + BWI76_RS23210 0.66 -1.2

Or see this region's nucleotide sequence