Experiment: KLEB25 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22765 and BWI76_RS22770 are separated by 346 nucleotides BWI76_RS22770 and BWI76_RS22775 overlap by 72 nucleotides BWI76_RS22775 and BWI76_RS22780 are separated by 56 nucleotides
BWI76_RS22765: BWI76_RS22765 - sulfite reductase subunit alpha, at 4,745,693 to 4,747,492
_RS22765
BWI76_RS22770: BWI76_RS22770 - 6-pyruvoyl tetrahydrobiopterin synthase, at 4,747,839 to 4,748,000
_RS22770
BWI76_RS22775: BWI76_RS22775 - 6-carboxytetrahydropterin synthase QueD, at 4,747,929 to 4,748,291
_RS22775
BWI76_RS22780: BWI76_RS22780 - 7-carboxy-7-deazaguanine synthase QueE, at 4,748,348 to 4,749,019
_RS22780
Position (kb)
4747
4748
4749 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4747.131 kb on - strand, within BWI76_RS22765 at 4747.134 kb on - strand, within BWI76_RS22765 at 4747.136 kb on - strand, within BWI76_RS22765 at 4747.136 kb on - strand, within BWI76_RS22765 at 4747.146 kb on - strand, within BWI76_RS22765 at 4747.218 kb on - strand, within BWI76_RS22765 at 4747.324 kb on + strand at 4747.332 kb on - strand at 4747.376 kb on - strand at 4747.376 kb on - strand at 4747.498 kb on + strand at 4747.519 kb on - strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.613 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.677 kb on + strand at 4747.692 kb on - strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.957 kb on + strand at 4747.968 kb on + strand at 4748.026 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.165 kb on + strand, within BWI76_RS22775 at 4748.187 kb on - strand, within BWI76_RS22775 at 4748.330 kb on - strand at 4748.401 kb on - strand at 4748.468 kb on - strand, within BWI76_RS22780 at 4748.605 kb on + strand, within BWI76_RS22780 at 4748.613 kb on - strand, within BWI76_RS22780 at 4748.627 kb on + strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.774 kb on - strand, within BWI76_RS22780 at 4748.779 kb on - strand, within BWI76_RS22780 at 4748.833 kb on + strand, within BWI76_RS22780 at 4748.884 kb on + strand, within BWI76_RS22780 at 4748.972 kb on + strand at 4748.974 kb on - strand at 4748.987 kb on + strand at 4748.988 kb on + strand at 4748.996 kb on - strand at 4749.014 kb on - strand at 4749.030 kb on + strand at 4749.068 kb on + strand at 4749.073 kb on + strand at 4749.073 kb on + strand at 4749.079 kb on + strand at 4749.081 kb on - strand at 4749.082 kb on - strand at 4749.084 kb on + strand at 4749.118 kb on + strand at 4749.126 kb on - strand at 4749.128 kb on + strand at 4749.134 kb on - strand at 4749.134 kb on - strand at 4749.156 kb on - strand at 4749.196 kb on + strand at 4749.204 kb on - strand at 4749.204 kb on - strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 0.1 remove 4,747,131 - BWI76_RS22765 0.80 +0.6 4,747,134 - BWI76_RS22765 0.80 +0.3 4,747,136 - BWI76_RS22765 0.80 -0.2 4,747,136 - BWI76_RS22765 0.80 -0.1 4,747,146 - BWI76_RS22765 0.81 -1.9 4,747,218 - BWI76_RS22765 0.85 -0.9 4,747,324 + -0.6 4,747,332 - -0.6 4,747,376 - +0.3 4,747,376 - +0.0 4,747,498 + +2.3 4,747,519 - -0.1 4,747,612 + -1.2 4,747,612 + +0.1 4,747,612 + -0.9 4,747,613 - -0.1 4,747,620 - -1.2 4,747,620 - +0.2 4,747,620 - -0.4 4,747,620 - +0.0 4,747,677 + -0.0 4,747,692 - -0.0 4,747,943 + -0.1 4,747,943 + +0.2 4,747,943 + -0.3 4,747,957 + -0.3 4,747,968 + +0.1 4,748,026 + BWI76_RS22775 0.27 -0.6 4,748,096 + BWI76_RS22775 0.46 -0.4 4,748,096 + BWI76_RS22775 0.46 -0.1 4,748,165 + BWI76_RS22775 0.65 -0.3 4,748,187 - BWI76_RS22775 0.71 -1.7 4,748,330 - +0.1 4,748,401 - -0.1 4,748,468 - BWI76_RS22780 0.18 -0.3 4,748,605 + BWI76_RS22780 0.38 +0.4 4,748,613 - BWI76_RS22780 0.39 +0.1 4,748,627 + BWI76_RS22780 0.42 +0.0 4,748,635 - BWI76_RS22780 0.43 -0.0 4,748,635 - BWI76_RS22780 0.43 -0.3 4,748,774 - BWI76_RS22780 0.63 -0.5 4,748,779 - BWI76_RS22780 0.64 -0.6 4,748,833 + BWI76_RS22780 0.72 -1.1 4,748,884 + BWI76_RS22780 0.80 -0.2 4,748,972 + -0.4 4,748,974 - -0.7 4,748,987 + -0.2 4,748,988 + -0.2 4,748,996 - -0.4 4,749,014 - -0.7 4,749,030 + +0.7 4,749,068 + +0.1 4,749,073 + -0.2 4,749,073 + -0.3 4,749,079 + -0.9 4,749,081 - -0.2 4,749,082 - +0.3 4,749,084 + -0.1 4,749,118 + -0.2 4,749,126 - -0.2 4,749,128 + +0.2 4,749,134 - -0.7 4,749,134 - -0.8 4,749,156 - +0.3 4,749,196 + -1.0 4,749,204 - -0.4 4,749,204 - +0.1 4,749,238 + -0.7 4,749,238 + -0.0 4,749,238 + -1.9 4,749,246 - +0.3 4,749,246 - -0.1 4,749,246 - -0.8 4,749,246 - -0.4
Or see this region's nucleotide sequence