Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24385 and BWI76_RS24390 are separated by 24 nucleotides BWI76_RS24390 and BWI76_RS24395 overlap by 4 nucleotides BWI76_RS24395 and BWI76_RS24400 are separated by 195 nucleotides
BWI76_RS24385: BWI76_RS24385 - phosphodiesterase, at 5,083,428 to 5,084,255
_RS24385
BWI76_RS24390: BWI76_RS24390 - hypothetical protein, at 5,084,280 to 5,084,702
_RS24390
BWI76_RS24395: BWI76_RS24395 - ADP-ribose diphosphatase, at 5,084,699 to 5,085,331
_RS24395
BWI76_RS24400: BWI76_RS24400 - outer membrane channel protein TolC, at 5,085,527 to 5,086,975
_RS24400
Position (kb) 5084
5085
5086 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5083.726 kb on - strand, within BWI76_RS24385 at 5083.778 kb on + strand, within BWI76_RS24385 at 5083.849 kb on - strand at 5083.945 kb on + strand, within BWI76_RS24385 at 5084.023 kb on + strand, within BWI76_RS24385 at 5084.183 kb on + strand at 5084.434 kb on - strand, within BWI76_RS24390 at 5084.593 kb on - strand, within BWI76_RS24390 at 5084.767 kb on - strand, within BWI76_RS24395 at 5084.799 kb on + strand, within BWI76_RS24395 at 5084.807 kb on - strand, within BWI76_RS24395 at 5084.953 kb on - strand, within BWI76_RS24395 at 5084.963 kb on - strand at 5084.963 kb on - strand, within BWI76_RS24395 at 5084.971 kb on + strand, within BWI76_RS24395 at 5085.101 kb on - strand, within BWI76_RS24395 at 5085.142 kb on - strand, within BWI76_RS24395 at 5085.195 kb on - strand, within BWI76_RS24395 at 5085.220 kb on + strand, within BWI76_RS24395 at 5085.309 kb on - strand at 5085.316 kb on + strand at 5085.337 kb on - strand at 5085.349 kb on - strand at 5085.462 kb on + strand at 5085.497 kb on + strand at 5085.505 kb on - strand at 5085.555 kb on + strand at 5085.555 kb on + strand at 5085.555 kb on + strand at 5085.563 kb on - strand at 5085.563 kb on - strand at 5085.563 kb on - strand at 5085.563 kb on - strand at 5085.567 kb on - strand at 5085.590 kb on + strand at 5085.633 kb on + strand at 5085.633 kb on + strand at 5085.633 kb on + strand at 5085.641 kb on - strand at 5085.682 kb on + strand, within BWI76_RS24400 at 5085.731 kb on + strand, within BWI76_RS24400 at 5085.731 kb on + strand, within BWI76_RS24400 at 5085.792 kb on + strand, within BWI76_RS24400 at 5085.872 kb on + strand, within BWI76_RS24400 at 5085.961 kb on + strand, within BWI76_RS24400 at 5085.969 kb on - strand, within BWI76_RS24400 at 5086.007 kb on - strand, within BWI76_RS24400 at 5086.142 kb on - strand, within BWI76_RS24400 at 5086.236 kb on - strand, within BWI76_RS24400 at 5086.322 kb on - strand, within BWI76_RS24400
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 5,083,726 - BWI76_RS24385 0.36 +0.3 5,083,778 + BWI76_RS24385 0.42 -1.0 5,083,849 - +2.6 5,083,945 + BWI76_RS24385 0.62 -0.2 5,084,023 + BWI76_RS24385 0.72 +0.0 5,084,183 + -1.6 5,084,434 - BWI76_RS24390 0.36 -0.3 5,084,593 - BWI76_RS24390 0.74 -0.5 5,084,767 - BWI76_RS24395 0.11 -0.8 5,084,799 + BWI76_RS24395 0.16 -0.9 5,084,807 - BWI76_RS24395 0.17 -0.7 5,084,953 - BWI76_RS24395 0.40 -1.2 5,084,963 - -0.6 5,084,963 - BWI76_RS24395 0.42 -0.6 5,084,971 + BWI76_RS24395 0.43 -1.2 5,085,101 - BWI76_RS24395 0.64 -1.2 5,085,142 - BWI76_RS24395 0.70 -0.3 5,085,195 - BWI76_RS24395 0.78 -0.0 5,085,220 + BWI76_RS24395 0.82 -1.0 5,085,309 - -0.2 5,085,316 + -0.6 5,085,337 - -0.0 5,085,349 - +0.5 5,085,462 + -0.2 5,085,497 + -2.4 5,085,505 - -1.6 5,085,555 + +1.2 5,085,555 + +2.6 5,085,555 + -0.1 5,085,563 - -1.7 5,085,563 - -1.4 5,085,563 - -1.4 5,085,563 - +0.1 5,085,567 - +0.6 5,085,590 + -0.4 5,085,633 + -0.3 5,085,633 + -0.5 5,085,633 + +0.2 5,085,641 - +3.2 5,085,682 + BWI76_RS24400 0.11 -0.5 5,085,731 + BWI76_RS24400 0.14 +0.0 5,085,731 + BWI76_RS24400 0.14 +1.6 5,085,792 + BWI76_RS24400 0.18 -1.0 5,085,872 + BWI76_RS24400 0.24 -0.4 5,085,961 + BWI76_RS24400 0.30 +1.0 5,085,969 - BWI76_RS24400 0.31 +0.6 5,086,007 - BWI76_RS24400 0.33 +1.5 5,086,142 - BWI76_RS24400 0.42 +0.4 5,086,236 - BWI76_RS24400 0.49 +0.5 5,086,322 - BWI76_RS24400 0.55 +1.6
Or see this region's nucleotide sequence