Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14445 and BWI76_RS14450 are separated by 132 nucleotides BWI76_RS14450 and BWI76_RS14455 overlap by 4 nucleotides BWI76_RS14455 and BWI76_RS14460 are separated by 132 nucleotides
BWI76_RS14445: BWI76_RS14445 - amino acid ABC transporter substrate-binding protein, at 3,014,503 to 3,015,255
_RS14445
BWI76_RS14450: BWI76_RS14450 - hypothetical protein, at 3,015,388 to 3,015,939
_RS14450
BWI76_RS14455: BWI76_RS14455 - aspartate aminotransferase family protein, at 3,015,936 to 3,017,279
_RS14455
BWI76_RS14460: BWI76_RS14460 - MurR/RpiR family transcriptional regulator, at 3,017,412 to 3,018,269
_RS14460
Position (kb)
3015
3016
3017
3018 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3014.955 kb on + strand, within BWI76_RS14445 at 3014.968 kb on + strand, within BWI76_RS14445 at 3014.975 kb on - strand, within BWI76_RS14445 at 3015.148 kb on + strand, within BWI76_RS14445 at 3015.149 kb on - strand, within BWI76_RS14445 at 3015.149 kb on - strand, within BWI76_RS14445 at 3015.216 kb on - strand at 3015.283 kb on + strand at 3015.291 kb on - strand at 3015.407 kb on - strand at 3015.407 kb on - strand at 3015.420 kb on + strand at 3015.420 kb on - strand at 3015.426 kb on - strand at 3015.426 kb on - strand at 3015.426 kb on - strand at 3015.599 kb on + strand, within BWI76_RS14450 at 3015.600 kb on + strand, within BWI76_RS14450 at 3015.607 kb on - strand, within BWI76_RS14450 at 3015.614 kb on + strand, within BWI76_RS14450 at 3015.617 kb on - strand, within BWI76_RS14450 at 3015.810 kb on + strand, within BWI76_RS14450 at 3015.818 kb on - strand, within BWI76_RS14450 at 3015.979 kb on + strand at 3016.046 kb on + strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.054 kb on - strand at 3016.180 kb on - strand, within BWI76_RS14455 at 3016.242 kb on + strand, within BWI76_RS14455 at 3016.431 kb on + strand, within BWI76_RS14455 at 3016.439 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.630 kb on - strand, within BWI76_RS14455 at 3017.085 kb on + strand, within BWI76_RS14455 at 3017.087 kb on - strand, within BWI76_RS14455 at 3017.187 kb on + strand at 3017.245 kb on - strand at 3017.972 kb on - strand, within BWI76_RS14460 at 3018.128 kb on + strand, within BWI76_RS14460 at 3018.128 kb on + strand, within BWI76_RS14460 at 3018.223 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 3,014,955 + BWI76_RS14445 0.60 -0.6 3,014,968 + BWI76_RS14445 0.62 +0.4 3,014,975 - BWI76_RS14445 0.63 +0.3 3,015,148 + BWI76_RS14445 0.86 +0.2 3,015,149 - BWI76_RS14445 0.86 +2.0 3,015,149 - BWI76_RS14445 0.86 +0.1 3,015,216 - -0.1 3,015,283 + -0.8 3,015,291 - -0.2 3,015,407 - -0.9 3,015,407 - +0.5 3,015,420 + -2.3 3,015,420 - -0.0 3,015,426 - +0.4 3,015,426 - +0.2 3,015,426 - -0.8 3,015,599 + BWI76_RS14450 0.38 -0.7 3,015,600 + BWI76_RS14450 0.38 -0.3 3,015,607 - BWI76_RS14450 0.40 +1.2 3,015,614 + BWI76_RS14450 0.41 -1.0 3,015,617 - BWI76_RS14450 0.41 -2.2 3,015,810 + BWI76_RS14450 0.76 -0.1 3,015,818 - BWI76_RS14450 0.78 +0.4 3,015,979 + -0.8 3,016,046 + -0.1 3,016,054 - +1.1 3,016,054 - +0.7 3,016,054 - -0.9 3,016,054 - -0.0 3,016,054 - -0.3 3,016,180 - BWI76_RS14455 0.18 -1.3 3,016,242 + BWI76_RS14455 0.23 -1.3 3,016,431 + BWI76_RS14455 0.37 +0.5 3,016,439 - BWI76_RS14455 0.37 -3.2 3,016,509 - BWI76_RS14455 0.43 -0.3 3,016,509 - BWI76_RS14455 0.43 -0.7 3,016,630 - BWI76_RS14455 0.52 -1.7 3,017,085 + BWI76_RS14455 0.85 -0.6 3,017,087 - BWI76_RS14455 0.86 -1.0 3,017,187 + -1.3 3,017,245 - +0.5 3,017,972 - BWI76_RS14460 0.65 +0.1 3,018,128 + BWI76_RS14460 0.83 -1.1 3,018,128 + BWI76_RS14460 0.83 +2.1 3,018,223 + +3.0
Or see this region's nucleotide sequence