Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14455

Experiment: KLEB25 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14445 and BWI76_RS14450 are separated by 132 nucleotidesBWI76_RS14450 and BWI76_RS14455 overlap by 4 nucleotidesBWI76_RS14455 and BWI76_RS14460 are separated by 132 nucleotides BWI76_RS14445: BWI76_RS14445 - amino acid ABC transporter substrate-binding protein, at 3,014,503 to 3,015,255 _RS14445 BWI76_RS14450: BWI76_RS14450 - hypothetical protein, at 3,015,388 to 3,015,939 _RS14450 BWI76_RS14455: BWI76_RS14455 - aspartate aminotransferase family protein, at 3,015,936 to 3,017,279 _RS14455 BWI76_RS14460: BWI76_RS14460 - MurR/RpiR family transcriptional regulator, at 3,017,412 to 3,018,269 _RS14460 Position (kb) 3015 3016 3017 3018Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3014.955 kb on + strand, within BWI76_RS14445at 3014.968 kb on + strand, within BWI76_RS14445at 3014.975 kb on - strand, within BWI76_RS14445at 3015.148 kb on + strand, within BWI76_RS14445at 3015.149 kb on - strand, within BWI76_RS14445at 3015.149 kb on - strand, within BWI76_RS14445at 3015.216 kb on - strandat 3015.283 kb on + strandat 3015.291 kb on - strandat 3015.407 kb on - strandat 3015.407 kb on - strandat 3015.420 kb on + strandat 3015.420 kb on - strandat 3015.426 kb on - strandat 3015.426 kb on - strandat 3015.426 kb on - strandat 3015.599 kb on + strand, within BWI76_RS14450at 3015.600 kb on + strand, within BWI76_RS14450at 3015.607 kb on - strand, within BWI76_RS14450at 3015.614 kb on + strand, within BWI76_RS14450at 3015.617 kb on - strand, within BWI76_RS14450at 3015.810 kb on + strand, within BWI76_RS14450at 3015.818 kb on - strand, within BWI76_RS14450at 3015.979 kb on + strandat 3016.046 kb on + strandat 3016.054 kb on - strandat 3016.054 kb on - strandat 3016.054 kb on - strandat 3016.054 kb on - strandat 3016.054 kb on - strandat 3016.180 kb on - strand, within BWI76_RS14455at 3016.242 kb on + strand, within BWI76_RS14455at 3016.431 kb on + strand, within BWI76_RS14455at 3016.439 kb on - strand, within BWI76_RS14455at 3016.509 kb on - strand, within BWI76_RS14455at 3016.509 kb on - strand, within BWI76_RS14455at 3016.630 kb on - strand, within BWI76_RS14455at 3017.085 kb on + strand, within BWI76_RS14455at 3017.087 kb on - strand, within BWI76_RS14455at 3017.187 kb on + strandat 3017.245 kb on - strandat 3017.972 kb on - strand, within BWI76_RS14460at 3018.128 kb on + strand, within BWI76_RS14460at 3018.128 kb on + strand, within BWI76_RS14460at 3018.223 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB25 MOI 1
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3,014,955 + BWI76_RS14445 0.60 -0.6
3,014,968 + BWI76_RS14445 0.62 +0.4
3,014,975 - BWI76_RS14445 0.63 +0.3
3,015,148 + BWI76_RS14445 0.86 +0.2
3,015,149 - BWI76_RS14445 0.86 +2.0
3,015,149 - BWI76_RS14445 0.86 +0.1
3,015,216 - -0.1
3,015,283 + -0.8
3,015,291 - -0.2
3,015,407 - -0.9
3,015,407 - +0.5
3,015,420 + -2.3
3,015,420 - -0.0
3,015,426 - +0.4
3,015,426 - +0.2
3,015,426 - -0.8
3,015,599 + BWI76_RS14450 0.38 -0.7
3,015,600 + BWI76_RS14450 0.38 -0.3
3,015,607 - BWI76_RS14450 0.40 +1.2
3,015,614 + BWI76_RS14450 0.41 -1.0
3,015,617 - BWI76_RS14450 0.41 -2.2
3,015,810 + BWI76_RS14450 0.76 -0.1
3,015,818 - BWI76_RS14450 0.78 +0.4
3,015,979 + -0.8
3,016,046 + -0.1
3,016,054 - +1.1
3,016,054 - +0.7
3,016,054 - -0.9
3,016,054 - -0.0
3,016,054 - -0.3
3,016,180 - BWI76_RS14455 0.18 -1.3
3,016,242 + BWI76_RS14455 0.23 -1.3
3,016,431 + BWI76_RS14455 0.37 +0.5
3,016,439 - BWI76_RS14455 0.37 -3.2
3,016,509 - BWI76_RS14455 0.43 -0.3
3,016,509 - BWI76_RS14455 0.43 -0.7
3,016,630 - BWI76_RS14455 0.52 -1.7
3,017,085 + BWI76_RS14455 0.85 -0.6
3,017,087 - BWI76_RS14455 0.86 -1.0
3,017,187 + -1.3
3,017,245 - +0.5
3,017,972 - BWI76_RS14460 0.65 +0.1
3,018,128 + BWI76_RS14460 0.83 -1.1
3,018,128 + BWI76_RS14460 0.83 +2.1
3,018,223 + +3.0

Or see this region's nucleotide sequence