Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14165 and BWI76_RS14170 are separated by 48 nucleotides BWI76_RS14170 and BWI76_RS14175 are separated by 135 nucleotides BWI76_RS14175 and BWI76_RS14180 overlap by 4 nucleotides
BWI76_RS14165: BWI76_RS14165 - trans-acting regulatory protein of aco operon, at 2,963,756 to 2,966,509
_RS14165
BWI76_RS14170: BWI76_RS14170 - hypothetical protein, at 2,966,558 to 2,966,941
_RS14170
BWI76_RS14175: BWI76_RS14175 - dehydrogenase, at 2,967,077 to 2,967,952
_RS14175
BWI76_RS14180: BWI76_RS14180 - hypothetical protein, at 2,967,949 to 2,969,034
_RS14180
Position (kb)
2967
2968 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2966.341 kb on + strand at 2966.408 kb on - strand at 2966.446 kb on + strand at 2966.561 kb on + strand at 2966.569 kb on - strand at 2966.579 kb on + strand at 2966.656 kb on + strand, within BWI76_RS14170 at 2966.741 kb on - strand, within BWI76_RS14170 at 2966.767 kb on - strand, within BWI76_RS14170 at 2966.840 kb on + strand, within BWI76_RS14170 at 2966.889 kb on - strand, within BWI76_RS14170 at 2966.907 kb on + strand at 2966.979 kb on + strand at 2966.979 kb on + strand at 2967.034 kb on - strand at 2967.036 kb on + strand at 2967.075 kb on + strand at 2967.075 kb on + strand at 2967.123 kb on + strand at 2967.147 kb on - strand at 2967.156 kb on + strand at 2967.220 kb on - strand, within BWI76_RS14175 at 2967.277 kb on + strand at 2967.285 kb on - strand, within BWI76_RS14175 at 2967.335 kb on - strand, within BWI76_RS14175 at 2967.571 kb on + strand, within BWI76_RS14175 at 2967.579 kb on - strand, within BWI76_RS14175 at 2967.708 kb on - strand, within BWI76_RS14175 at 2968.229 kb on - strand, within BWI76_RS14180 at 2968.331 kb on - strand, within BWI76_RS14180 at 2968.336 kb on - strand, within BWI76_RS14180 at 2968.401 kb on - strand, within BWI76_RS14180 at 2968.507 kb on - strand, within BWI76_RS14180 at 2968.519 kb on + strand, within BWI76_RS14180 at 2968.603 kb on - strand, within BWI76_RS14180 at 2968.694 kb on - strand, within BWI76_RS14180
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 2,966,341 + -2.4 2,966,408 - -0.1 2,966,446 + -0.2 2,966,561 + -0.8 2,966,569 - -0.2 2,966,579 + -0.2 2,966,656 + BWI76_RS14170 0.26 -0.4 2,966,741 - BWI76_RS14170 0.48 -0.4 2,966,767 - BWI76_RS14170 0.54 -0.2 2,966,840 + BWI76_RS14170 0.73 -0.2 2,966,889 - BWI76_RS14170 0.86 +1.2 2,966,907 + +0.2 2,966,979 + -0.3 2,966,979 + -2.2 2,967,034 - -0.9 2,967,036 + -0.5 2,967,075 + -1.7 2,967,075 + -1.9 2,967,123 + -0.9 2,967,147 - -0.1 2,967,156 + +0.1 2,967,220 - BWI76_RS14175 0.16 -1.4 2,967,277 + +4.0 2,967,285 - BWI76_RS14175 0.24 -0.4 2,967,335 - BWI76_RS14175 0.29 -2.1 2,967,571 + BWI76_RS14175 0.56 -0.6 2,967,579 - BWI76_RS14175 0.57 -0.1 2,967,708 - BWI76_RS14175 0.72 -0.7 2,968,229 - BWI76_RS14180 0.26 -0.4 2,968,331 - BWI76_RS14180 0.35 -0.2 2,968,336 - BWI76_RS14180 0.36 -0.7 2,968,401 - BWI76_RS14180 0.42 -0.2 2,968,507 - BWI76_RS14180 0.51 -3.0 2,968,519 + BWI76_RS14180 0.52 +0.0 2,968,603 - BWI76_RS14180 0.60 -0.7 2,968,694 - BWI76_RS14180 0.69 -0.0
Or see this region's nucleotide sequence