Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13440 and BWI76_RS13445 are separated by 72 nucleotides BWI76_RS13445 and BWI76_RS13450 are separated by 80 nucleotides BWI76_RS13450 and BWI76_RS13455 are separated by 3 nucleotides
BWI76_RS13440: BWI76_RS13440 - DUF2566 domain-containing protein, at 2,803,124 to 2,803,297
_RS13440
BWI76_RS13445: BWI76_RS13445 - alpha/beta hydrolase, at 2,803,370 to 2,804,140
_RS13445
BWI76_RS13450: BWI76_RS13450 - acyl-CoA thioesterase, at 2,804,221 to 2,805,477
_RS13450
BWI76_RS13455: BWI76_RS13455 - salicylaldehyde dehydrogenase, at 2,805,481 to 2,806,914
_RS13455
Position (kb)
2804
2805
2806 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2803.388 kb on + strand at 2803.593 kb on - strand, within BWI76_RS13445 at 2803.782 kb on - strand, within BWI76_RS13445 at 2804.244 kb on - strand at 2804.264 kb on - strand at 2804.306 kb on + strand at 2804.578 kb on + strand, within BWI76_RS13450 at 2804.645 kb on + strand, within BWI76_RS13450 at 2804.797 kb on + strand, within BWI76_RS13450 at 2804.846 kb on - strand, within BWI76_RS13450 at 2804.846 kb on - strand, within BWI76_RS13450 at 2805.218 kb on + strand at 2805.251 kb on - strand, within BWI76_RS13450 at 2805.798 kb on + strand, within BWI76_RS13455 at 2806.050 kb on - strand, within BWI76_RS13455 at 2806.363 kb on + strand, within BWI76_RS13455 at 2806.436 kb on - strand, within BWI76_RS13455
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 2,803,388 + -0.1 2,803,593 - BWI76_RS13445 0.29 +3.7 2,803,782 - BWI76_RS13445 0.53 +0.6 2,804,244 - +0.3 2,804,264 - -1.3 2,804,306 + +0.5 2,804,578 + BWI76_RS13450 0.28 -1.8 2,804,645 + BWI76_RS13450 0.34 -0.9 2,804,797 + BWI76_RS13450 0.46 -0.3 2,804,846 - BWI76_RS13450 0.50 -0.5 2,804,846 - BWI76_RS13450 0.50 -0.2 2,805,218 + +0.1 2,805,251 - BWI76_RS13450 0.82 -0.7 2,805,798 + BWI76_RS13455 0.22 +0.3 2,806,050 - BWI76_RS13455 0.40 +0.4 2,806,363 + BWI76_RS13455 0.62 +0.0 2,806,436 - BWI76_RS13455 0.67 -0.4
Or see this region's nucleotide sequence