Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13450

Experiment: KLEB25 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13440 and BWI76_RS13445 are separated by 72 nucleotidesBWI76_RS13445 and BWI76_RS13450 are separated by 80 nucleotidesBWI76_RS13450 and BWI76_RS13455 are separated by 3 nucleotides BWI76_RS13440: BWI76_RS13440 - DUF2566 domain-containing protein, at 2,803,124 to 2,803,297 _RS13440 BWI76_RS13445: BWI76_RS13445 - alpha/beta hydrolase, at 2,803,370 to 2,804,140 _RS13445 BWI76_RS13450: BWI76_RS13450 - acyl-CoA thioesterase, at 2,804,221 to 2,805,477 _RS13450 BWI76_RS13455: BWI76_RS13455 - salicylaldehyde dehydrogenase, at 2,805,481 to 2,806,914 _RS13455 Position (kb) 2804 2805 2806Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2803.388 kb on + strandat 2803.593 kb on - strand, within BWI76_RS13445at 2803.782 kb on - strand, within BWI76_RS13445at 2804.244 kb on - strandat 2804.264 kb on - strandat 2804.306 kb on + strandat 2804.578 kb on + strand, within BWI76_RS13450at 2804.645 kb on + strand, within BWI76_RS13450at 2804.797 kb on + strand, within BWI76_RS13450at 2804.846 kb on - strand, within BWI76_RS13450at 2804.846 kb on - strand, within BWI76_RS13450at 2805.218 kb on + strandat 2805.251 kb on - strand, within BWI76_RS13450at 2805.798 kb on + strand, within BWI76_RS13455at 2806.050 kb on - strand, within BWI76_RS13455at 2806.363 kb on + strand, within BWI76_RS13455at 2806.436 kb on - strand, within BWI76_RS13455

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB25 MOI 1
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2,803,388 + -0.1
2,803,593 - BWI76_RS13445 0.29 +3.7
2,803,782 - BWI76_RS13445 0.53 +0.6
2,804,244 - +0.3
2,804,264 - -1.3
2,804,306 + +0.5
2,804,578 + BWI76_RS13450 0.28 -1.8
2,804,645 + BWI76_RS13450 0.34 -0.9
2,804,797 + BWI76_RS13450 0.46 -0.3
2,804,846 - BWI76_RS13450 0.50 -0.5
2,804,846 - BWI76_RS13450 0.50 -0.2
2,805,218 + +0.1
2,805,251 - BWI76_RS13450 0.82 -0.7
2,805,798 + BWI76_RS13455 0.22 +0.3
2,806,050 - BWI76_RS13455 0.40 +0.4
2,806,363 + BWI76_RS13455 0.62 +0.0
2,806,436 - BWI76_RS13455 0.67 -0.4

Or see this region's nucleotide sequence