Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07780 and BWI76_RS07785 overlap by 4 nucleotides BWI76_RS07785 and BWI76_RS07790 overlap by 20 nucleotides BWI76_RS07790 and BWI76_RS07795 are separated by 180 nucleotides BWI76_RS07795 and BWI76_RS07800 are separated by 160 nucleotides
BWI76_RS07780: BWI76_RS07780 - hypothetical protein, at 1,650,686 to 1,650,883
_RS07780
BWI76_RS07785: BWI76_RS07785 - transcriptional regulator, at 1,650,880 to 1,651,284
_RS07785
BWI76_RS07790: BWI76_RS07790 - plasmid stabilization protein, at 1,651,265 to 1,651,566
_RS07790
BWI76_RS07795: BWI76_RS07795 - oxidoreductase, at 1,651,747 to 1,652,835
_RS07795
BWI76_RS07800: BWI76_RS07800 - ABC transporter, at 1,652,996 to 1,654,498
_RS07800
Position (kb) 1651
1652
1653 Strain fitness (log2 ratio) -2
-1
0
1 at 1650.773 kb on - strand, within BWI76_RS07780 at 1650.886 kb on - strand at 1650.966 kb on + strand, within BWI76_RS07785 at 1651.005 kb on + strand, within BWI76_RS07785 at 1651.288 kb on - strand at 1651.430 kb on - strand, within BWI76_RS07790 at 1651.827 kb on + strand at 1652.061 kb on - strand, within BWI76_RS07795 at 1652.160 kb on - strand, within BWI76_RS07795 at 1652.161 kb on - strand, within BWI76_RS07795 at 1652.237 kb on - strand, within BWI76_RS07795 at 1652.552 kb on - strand, within BWI76_RS07795 at 1652.711 kb on + strand, within BWI76_RS07795 at 1652.719 kb on - strand, within BWI76_RS07795 at 1652.724 kb on - strand, within BWI76_RS07795 at 1652.747 kb on - strand at 1653.212 kb on - strand, within BWI76_RS07800 at 1653.261 kb on - strand, within BWI76_RS07800 at 1653.289 kb on - strand, within BWI76_RS07800 at 1653.573 kb on - strand, within BWI76_RS07800 at 1653.717 kb on + strand, within BWI76_RS07800 at 1653.725 kb on - strand, within BWI76_RS07800 at 1653.762 kb on + strand, within BWI76_RS07800 at 1653.762 kb on + strand, within BWI76_RS07800 at 1653.770 kb on - strand, within BWI76_RS07800
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 1,650,773 - BWI76_RS07780 0.44 -0.1 1,650,886 - -0.6 1,650,966 + BWI76_RS07785 0.21 +0.4 1,651,005 + BWI76_RS07785 0.31 -0.5 1,651,288 - +0.9 1,651,430 - BWI76_RS07790 0.55 -0.2 1,651,827 + -0.0 1,652,061 - BWI76_RS07795 0.29 -0.6 1,652,160 - BWI76_RS07795 0.38 +0.3 1,652,161 - BWI76_RS07795 0.38 -0.6 1,652,237 - BWI76_RS07795 0.45 -0.9 1,652,552 - BWI76_RS07795 0.74 -1.0 1,652,711 + BWI76_RS07795 0.89 -1.5 1,652,719 - BWI76_RS07795 0.89 -1.1 1,652,724 - BWI76_RS07795 0.90 -0.3 1,652,747 - -0.1 1,653,212 - BWI76_RS07800 0.14 -2.0 1,653,261 - BWI76_RS07800 0.18 -0.3 1,653,289 - BWI76_RS07800 0.19 -0.2 1,653,573 - BWI76_RS07800 0.38 +0.3 1,653,717 + BWI76_RS07800 0.48 +0.7 1,653,725 - BWI76_RS07800 0.49 -0.1 1,653,762 + BWI76_RS07800 0.51 -0.9 1,653,762 + BWI76_RS07800 0.51 -0.6 1,653,770 - BWI76_RS07800 0.51 +0.1
Or see this region's nucleotide sequence