Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02820 and BWI76_RS02825 are separated by 64 nucleotides BWI76_RS02825 and BWI76_RS02830 are separated by 162 nucleotides
BWI76_RS02820: BWI76_RS02820 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 576,022 to 577,395
_RS02820
BWI76_RS02825: BWI76_RS02825 - putative acyltransferase domain protein, at 577,460 to 578,005
_RS02825
BWI76_RS02830: BWI76_RS02830 - DUF4432 domain-containing protein, at 578,168 to 579,376
_RS02830
Position (kb) 577
578
579 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 576.546 kb on + strand, within BWI76_RS02820 at 576.731 kb on + strand, within BWI76_RS02820 at 576.739 kb on - strand, within BWI76_RS02820 at 576.792 kb on + strand, within BWI76_RS02820 at 576.909 kb on - strand, within BWI76_RS02820 at 577.147 kb on - strand, within BWI76_RS02820 at 577.269 kb on + strand at 577.396 kb on + strand at 577.522 kb on + strand, within BWI76_RS02825 at 577.528 kb on - strand, within BWI76_RS02825 at 577.552 kb on + strand, within BWI76_RS02825 at 577.560 kb on - strand, within BWI76_RS02825 at 577.634 kb on + strand, within BWI76_RS02825 at 577.911 kb on + strand, within BWI76_RS02825 at 578.213 kb on + strand at 578.264 kb on + strand at 578.272 kb on + strand at 578.272 kb on - strand at 578.277 kb on - strand at 578.282 kb on - strand at 578.307 kb on + strand, within BWI76_RS02830 at 578.358 kb on - strand, within BWI76_RS02830 at 578.425 kb on + strand, within BWI76_RS02830 at 578.623 kb on + strand, within BWI76_RS02830 at 578.732 kb on + strand, within BWI76_RS02830 at 578.920 kb on + strand, within BWI76_RS02830 at 578.920 kb on + strand, within BWI76_RS02830 at 578.921 kb on + strand, within BWI76_RS02830 at 578.922 kb on + strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830 at 578.930 kb on - strand, within BWI76_RS02830
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 576,546 + BWI76_RS02820 0.38 -0.7 576,731 + BWI76_RS02820 0.52 -0.4 576,739 - BWI76_RS02820 0.52 +0.0 576,792 + BWI76_RS02820 0.56 -0.2 576,909 - BWI76_RS02820 0.65 -0.2 577,147 - BWI76_RS02820 0.82 -0.6 577,269 + -3.1 577,396 + +0.0 577,522 + BWI76_RS02825 0.11 -0.8 577,528 - BWI76_RS02825 0.12 -0.7 577,552 + BWI76_RS02825 0.17 -0.8 577,560 - BWI76_RS02825 0.18 -0.4 577,634 + BWI76_RS02825 0.32 -0.3 577,911 + BWI76_RS02825 0.83 -1.1 578,213 + -0.0 578,264 + -0.5 578,272 + -0.6 578,272 - -1.0 578,277 - -0.9 578,282 - -0.5 578,307 + BWI76_RS02830 0.11 -0.1 578,358 - BWI76_RS02830 0.16 +0.4 578,425 + BWI76_RS02830 0.21 -0.8 578,623 + BWI76_RS02830 0.38 -0.4 578,732 + BWI76_RS02830 0.47 -0.4 578,920 + BWI76_RS02830 0.62 -0.4 578,920 + BWI76_RS02830 0.62 +0.5 578,921 + BWI76_RS02830 0.62 +0.1 578,922 + BWI76_RS02830 0.62 -0.1 578,930 - BWI76_RS02830 0.63 +0.0 578,930 - BWI76_RS02830 0.63 -0.7 578,930 - BWI76_RS02830 0.63 -1.1 578,930 - BWI76_RS02830 0.63 -0.0
Or see this region's nucleotide sequence