Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27105

Experiment: KLEB25 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27100 and BWI76_RS27105 overlap by 4 nucleotidesBWI76_RS27105 and BWI76_RS27110 overlap by 8 nucleotidesBWI76_RS27110 and BWI76_RS27115 overlap by 7 nucleotides BWI76_RS27100: BWI76_RS27100 - carbohydrate kinase, at 5,623,536 to 5,625,041 _RS27100 BWI76_RS27105: BWI76_RS27105 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 5,625,038 to 5,625,700 _RS27105 BWI76_RS27110: BWI76_RS27110 - xylulose 5-phosphate 3-epimerase, at 5,625,693 to 5,626,553 _RS27110 BWI76_RS27115: BWI76_RS27115 - L-ribulose-5-phosphate 4-epimerase, at 5,626,547 to 5,627,245 _RS27115 Position (kb) 5625 5626Strain fitness (log2 ratio) -1 0 1at 5624.175 kb on + strand, within BWI76_RS27100at 5624.183 kb on - strand, within BWI76_RS27100at 5624.241 kb on - strand, within BWI76_RS27100at 5624.281 kb on + strand, within BWI76_RS27100at 5624.377 kb on + strand, within BWI76_RS27100at 5624.390 kb on + strand, within BWI76_RS27100at 5624.398 kb on - strand, within BWI76_RS27100at 5624.405 kb on - strand, within BWI76_RS27100at 5624.474 kb on + strand, within BWI76_RS27100at 5624.603 kb on - strand, within BWI76_RS27100at 5624.609 kb on - strand, within BWI76_RS27100at 5624.872 kb on + strand, within BWI76_RS27100at 5624.879 kb on - strand, within BWI76_RS27100at 5624.944 kb on + strandat 5625.000 kb on + strandat 5625.000 kb on + strandat 5625.013 kb on - strandat 5625.019 kb on + strandat 5625.079 kb on + strandat 5625.099 kb on + strandat 5625.349 kb on - strand, within BWI76_RS27105at 5625.416 kb on - strand, within BWI76_RS27105at 5625.458 kb on + strand, within BWI76_RS27105at 5625.475 kb on + strand, within BWI76_RS27105at 5625.525 kb on + strand, within BWI76_RS27105at 5625.549 kb on + strand, within BWI76_RS27105at 5625.557 kb on - strand, within BWI76_RS27105at 5625.582 kb on - strand, within BWI76_RS27105at 5625.590 kb on + strand, within BWI76_RS27105at 5625.619 kb on + strand, within BWI76_RS27105at 5625.743 kb on + strandat 5625.756 kb on - strandat 5625.852 kb on - strand, within BWI76_RS27110at 5625.869 kb on - strand, within BWI76_RS27110at 5625.947 kb on + strand, within BWI76_RS27110at 5625.991 kb on - strand, within BWI76_RS27110at 5626.103 kb on - strand, within BWI76_RS27110at 5626.118 kb on + strandat 5626.258 kb on - strandat 5626.334 kb on + strand, within BWI76_RS27110at 5626.351 kb on + strand, within BWI76_RS27110at 5626.351 kb on + strand, within BWI76_RS27110at 5626.508 kb on + strandat 5626.614 kb on + strandat 5626.614 kb on + strandat 5626.686 kb on + strand, within BWI76_RS27115at 5626.686 kb on + strand, within BWI76_RS27115at 5626.686 kb on + strand, within BWI76_RS27115at 5626.693 kb on + strand, within BWI76_RS27115

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB25 MOI 1
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5,624,175 + BWI76_RS27100 0.42 -0.1
5,624,183 - BWI76_RS27100 0.43 -0.3
5,624,241 - BWI76_RS27100 0.47 -0.8
5,624,281 + BWI76_RS27100 0.49 +0.5
5,624,377 + BWI76_RS27100 0.56 -0.8
5,624,390 + BWI76_RS27100 0.57 -0.4
5,624,398 - BWI76_RS27100 0.57 -0.3
5,624,405 - BWI76_RS27100 0.58 -0.1
5,624,474 + BWI76_RS27100 0.62 -0.5
5,624,603 - BWI76_RS27100 0.71 +0.5
5,624,609 - BWI76_RS27100 0.71 +0.3
5,624,872 + BWI76_RS27100 0.89 -0.1
5,624,879 - BWI76_RS27100 0.89 -0.7
5,624,944 + -0.4
5,625,000 + -1.5
5,625,000 + -0.6
5,625,013 - +0.1
5,625,019 + -0.4
5,625,079 + +0.1
5,625,099 + -1.1
5,625,349 - BWI76_RS27105 0.47 -0.7
5,625,416 - BWI76_RS27105 0.57 +0.0
5,625,458 + BWI76_RS27105 0.63 -0.1
5,625,475 + BWI76_RS27105 0.66 -0.3
5,625,525 + BWI76_RS27105 0.73 -0.2
5,625,549 + BWI76_RS27105 0.77 -1.0
5,625,557 - BWI76_RS27105 0.78 -0.6
5,625,582 - BWI76_RS27105 0.82 -1.3
5,625,590 + BWI76_RS27105 0.83 -0.1
5,625,619 + BWI76_RS27105 0.88 +0.0
5,625,743 + +0.1
5,625,756 - -0.9
5,625,852 - BWI76_RS27110 0.18 -1.7
5,625,869 - BWI76_RS27110 0.20 -0.5
5,625,947 + BWI76_RS27110 0.30 -0.9
5,625,991 - BWI76_RS27110 0.35 -0.3
5,626,103 - BWI76_RS27110 0.48 -0.3
5,626,118 + -0.7
5,626,258 - -0.9
5,626,334 + BWI76_RS27110 0.74 -1.0
5,626,351 + BWI76_RS27110 0.76 +0.3
5,626,351 + BWI76_RS27110 0.76 -0.8
5,626,508 + -1.0
5,626,614 + -0.1
5,626,614 + +0.4
5,626,686 + BWI76_RS27115 0.20 -0.1
5,626,686 + BWI76_RS27115 0.20 -0.0
5,626,686 + BWI76_RS27115 0.20 +0.5
5,626,693 + BWI76_RS27115 0.21 -0.2

Or see this region's nucleotide sequence