Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18600 and BWI76_RS18605 overlap by 11 nucleotides BWI76_RS18605 and BWI76_RS18610 are separated by 178 nucleotides BWI76_RS18610 and BWI76_RS18615 are separated by 213 nucleotides BWI76_RS18615 and BWI76_RS18620 are separated by 102 nucleotides
BWI76_RS18600: BWI76_RS18600 - HupC/HyaC/HydC family protein, at 3,857,352 to 3,857,960
_RS18600
BWI76_RS18605: BWI76_RS18605 - oxidoreductase molybdopterin-binding protein, at 3,857,950 to 3,858,717
_RS18605
BWI76_RS18610: BWI76_RS18610 - transcriptional regulator LysR, at 3,858,896 to 3,859,882
_RS18610
BWI76_RS18615: BWI76_RS18615 - glutathione S-transferase domain protein, at 3,860,096 to 3,860,704
_RS18615
BWI76_RS18620: BWI76_RS18620 - L,D-transpeptidase, at 3,860,807 to 3,861,748
_RS18620
Position (kb)
3858
3859
3860 Strain fitness (log2 ratio)
-2
-1
0
1 at 3858.065 kb on + strand, within BWI76_RS18605 at 3858.180 kb on + strand, within BWI76_RS18605 at 3858.189 kb on - strand, within BWI76_RS18605 at 3858.685 kb on + strand at 3859.038 kb on + strand, within BWI76_RS18610 at 3859.334 kb on + strand, within BWI76_RS18610 at 3859.580 kb on - strand, within BWI76_RS18610 at 3859.677 kb on - strand, within BWI76_RS18610 at 3859.972 kb on + strand at 3860.006 kb on + strand at 3860.006 kb on + strand at 3860.014 kb on + strand at 3860.016 kb on - strand at 3860.038 kb on - strand at 3860.338 kb on + strand, within BWI76_RS18615 at 3860.362 kb on + strand, within BWI76_RS18615 at 3860.387 kb on - strand, within BWI76_RS18615 at 3860.477 kb on - strand, within BWI76_RS18615 at 3860.492 kb on - strand, within BWI76_RS18615 at 3860.520 kb on - strand, within BWI76_RS18615 at 3860.558 kb on - strand, within BWI76_RS18615 at 3860.560 kb on + strand, within BWI76_RS18615 at 3860.617 kb on + strand at 3860.705 kb on + strand at 3860.713 kb on - strand at 3860.757 kb on - strand at 3860.867 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 3,858,065 + BWI76_RS18605 0.15 -2.4 3,858,180 + BWI76_RS18605 0.30 +0.8 3,858,189 - BWI76_RS18605 0.31 +0.9 3,858,685 + -0.4 3,859,038 + BWI76_RS18610 0.14 +0.1 3,859,334 + BWI76_RS18610 0.44 -2.2 3,859,580 - BWI76_RS18610 0.69 -1.9 3,859,677 - BWI76_RS18610 0.79 -0.7 3,859,972 + +0.3 3,860,006 + -0.5 3,860,006 + +0.8 3,860,014 + -0.3 3,860,016 - -0.8 3,860,038 - -0.6 3,860,338 + BWI76_RS18615 0.40 -0.4 3,860,362 + BWI76_RS18615 0.44 -1.4 3,860,387 - BWI76_RS18615 0.48 -0.4 3,860,477 - BWI76_RS18615 0.63 +0.2 3,860,492 - BWI76_RS18615 0.65 +0.1 3,860,520 - BWI76_RS18615 0.70 -0.4 3,860,558 - BWI76_RS18615 0.76 +1.0 3,860,560 + BWI76_RS18615 0.76 +0.4 3,860,617 + -1.0 3,860,705 + -1.1 3,860,713 - +0.2 3,860,757 - -0.4 3,860,867 + +1.4
Or see this region's nucleotide sequence