Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18410 and BWI76_RS18415 overlap by 26 nucleotides BWI76_RS18415 and BWI76_RS18420 overlap by 20 nucleotides
BWI76_RS18410: BWI76_RS18410 - drug/metabolite exporter YedA, at 3,820,405 to 3,821,316
_RS18410
BWI76_RS18415: BWI76_RS18415 - very short patch repair endonuclease, at 3,821,291 to 3,821,785
_RS18415
BWI76_RS18420: BWI76_RS18420 - DNA (cytosine-5-)-methyltransferase, at 3,821,766 to 3,823,238
_RS18420
Position (kb)
3821
3822 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3820.390 kb on - strand at 3820.679 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3820.848 kb on + strand, within BWI76_RS18410 at 3821.203 kb on + strand, within BWI76_RS18410 at 3821.326 kb on + strand at 3821.438 kb on + strand, within BWI76_RS18415 at 3821.517 kb on + strand, within BWI76_RS18415 at 3821.895 kb on + strand at 3822.162 kb on + strand, within BWI76_RS18420 at 3822.213 kb on - strand, within BWI76_RS18420 at 3822.238 kb on + strand, within BWI76_RS18420 at 3822.273 kb on + strand, within BWI76_RS18420 at 3822.299 kb on + strand, within BWI76_RS18420 at 3822.317 kb on + strand, within BWI76_RS18420 at 3822.344 kb on + strand, within BWI76_RS18420 at 3822.431 kb on + strand, within BWI76_RS18420 at 3822.439 kb on - strand, within BWI76_RS18420 at 3822.713 kb on - strand, within BWI76_RS18420
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 3,820,390 - +0.5 3,820,679 + BWI76_RS18410 0.30 -0.1 3,820,848 + BWI76_RS18410 0.49 +0.2 3,820,848 + BWI76_RS18410 0.49 +1.1 3,820,848 + BWI76_RS18410 0.49 -3.0 3,820,848 + BWI76_RS18410 0.49 -0.9 3,821,203 + BWI76_RS18410 0.88 +1.3 3,821,326 + +2.9 3,821,438 + BWI76_RS18415 0.30 -0.8 3,821,517 + BWI76_RS18415 0.46 -0.7 3,821,895 + -0.2 3,822,162 + BWI76_RS18420 0.27 -0.0 3,822,213 - BWI76_RS18420 0.30 +0.2 3,822,238 + BWI76_RS18420 0.32 -0.1 3,822,273 + BWI76_RS18420 0.34 -0.8 3,822,299 + BWI76_RS18420 0.36 +0.8 3,822,317 + BWI76_RS18420 0.37 -0.3 3,822,344 + BWI76_RS18420 0.39 +0.1 3,822,431 + BWI76_RS18420 0.45 -0.6 3,822,439 - BWI76_RS18420 0.46 -1.0 3,822,713 - BWI76_RS18420 0.64 -0.0
Or see this region's nucleotide sequence