Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15180 and BWI76_RS15185 are separated by 111 nucleotides BWI76_RS15185 and BWI76_RS15190 are separated by 90 nucleotides BWI76_RS15190 and BWI76_RS15195 are separated by 107 nucleotides BWI76_RS15195 and BWI76_RS15200 overlap by 1 nucleotides
BWI76_RS15180: BWI76_RS15180 - altronate oxidoreductase, at 3,181,934 to 3,183,385
_RS15180
BWI76_RS15185: BWI76_RS15185 - N-acetyltransferase, at 3,183,497 to 3,184,027
_RS15185
BWI76_RS15190: BWI76_RS15190 - GGDEF domain-containing protein, at 3,184,118 to 3,185,530
_RS15190
BWI76_RS15195: BWI76_RS15195 - DUF4186 domain-containing protein, at 3,185,638 to 3,186,012
_RS15195
BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938
_RS15200
Position (kb)
3184
3185
3186 Strain fitness (log2 ratio)
-2
-1
0
1 at 3183.141 kb on + strand at 3183.141 kb on + strand, within BWI76_RS15180 at 3183.345 kb on - strand at 3183.648 kb on + strand, within BWI76_RS15185 at 3183.910 kb on + strand, within BWI76_RS15185 at 3184.420 kb on + strand, within BWI76_RS15190 at 3184.503 kb on - strand, within BWI76_RS15190 at 3184.511 kb on + strand, within BWI76_RS15190 at 3184.607 kb on + strand, within BWI76_RS15190 at 3184.979 kb on - strand, within BWI76_RS15190 at 3185.286 kb on - strand, within BWI76_RS15190 at 3185.294 kb on - strand, within BWI76_RS15190 at 3185.446 kb on + strand at 3185.446 kb on + strand at 3185.543 kb on + strand at 3185.710 kb on - strand, within BWI76_RS15195 at 3185.881 kb on - strand, within BWI76_RS15195 at 3186.093 kb on - strand at 3186.326 kb on + strand, within BWI76_RS15200
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 3,183,141 + +0.5 3,183,141 + BWI76_RS15180 0.83 +0.1 3,183,345 - -0.1 3,183,648 + BWI76_RS15185 0.28 +1.5 3,183,910 + BWI76_RS15185 0.78 -0.5 3,184,420 + BWI76_RS15190 0.21 +0.1 3,184,503 - BWI76_RS15190 0.27 -0.4 3,184,511 + BWI76_RS15190 0.28 +0.1 3,184,607 + BWI76_RS15190 0.35 -1.9 3,184,979 - BWI76_RS15190 0.61 -0.4 3,185,286 - BWI76_RS15190 0.83 -2.2 3,185,294 - BWI76_RS15190 0.83 -0.6 3,185,446 + +1.6 3,185,446 + -0.7 3,185,543 + +0.2 3,185,710 - BWI76_RS15195 0.19 -1.4 3,185,881 - BWI76_RS15195 0.65 -0.6 3,186,093 - -0.5 3,186,326 + BWI76_RS15200 0.34 -0.0
Or see this region's nucleotide sequence