Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14770 and BWI76_RS14775 overlap by 8 nucleotides BWI76_RS14775 and BWI76_RS14780 are separated by 9 nucleotides BWI76_RS14780 and BWI76_RS14785 are separated by 435 nucleotides BWI76_RS14785 and BWI76_RS14790 are separated by 36 nucleotides
BWI76_RS14770: BWI76_RS14770 - PTS IIA 2 domain protein, at 3,098,331 to 3,098,774
_RS14770
BWI76_RS14775: BWI76_RS14775 - creatinine amidohydrolase, at 3,098,767 to 3,099,507
_RS14775
BWI76_RS14780: BWI76_RS14780 - phosphotriesterase, at 3,099,517 to 3,100,488
_RS14780
BWI76_RS14785: BWI76_RS14785 - hypothetical protein, at 3,100,924 to 3,101,172
_RS14785
BWI76_RS14790: BWI76_RS14790 - 2-dehydropantoate 2-reductase, at 3,101,209 to 3,102,102
_RS14790
Position (kb) 3099
3100
3101 Strain fitness (log2 ratio) -2
-1
0
1 at 3098.731 kb on - strand at 3098.906 kb on + strand, within BWI76_RS14775 at 3099.126 kb on + strand at 3099.191 kb on + strand, within BWI76_RS14775 at 3099.743 kb on + strand, within BWI76_RS14780 at 3100.077 kb on + strand, within BWI76_RS14780 at 3100.176 kb on - strand, within BWI76_RS14780 at 3100.627 kb on + strand at 3101.321 kb on - strand at 3101.463 kb on + strand, within BWI76_RS14790
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 3,098,731 - -1.8 3,098,906 + BWI76_RS14775 0.19 +0.4 3,099,126 + +1.6 3,099,191 + BWI76_RS14775 0.57 +0.8 3,099,743 + BWI76_RS14780 0.23 -0.4 3,100,077 + BWI76_RS14780 0.58 +0.5 3,100,176 - BWI76_RS14780 0.68 -1.9 3,100,627 + +0.3 3,101,321 - -0.4 3,101,463 + BWI76_RS14790 0.28 -0.6
Or see this region's nucleotide sequence