Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14390 and BWI76_RS14395 are separated by 517 nucleotides BWI76_RS14395 and BWI76_RS14400 are separated by 110 nucleotides BWI76_RS14400 and BWI76_RS14405 are separated by 120 nucleotides BWI76_RS14405 and BWI76_RS14410 are separated by 91 nucleotides BWI76_RS14410 and BWI76_RS14415 are separated by 118 nucleotides
BWI76_RS14390: BWI76_RS14390 - hypothetical protein, at 3,006,987 to 3,007,862
_RS14390
BWI76_RS14395: BWI76_RS14395 - hypothetical protein, at 3,008,380 to 3,008,517
_RS14395
BWI76_RS14400: BWI76_RS14400 - ISNCY family transposase, at 3,008,628 to 3,009,198
_RS14400
BWI76_RS14405: BWI76_RS14405 - steroid Delta-isomerase, at 3,009,319 to 3,009,672
_RS14405
BWI76_RS14410: BWI76_RS14410 - hypothetical protein, at 3,009,764 to 3,009,967
_RS14410
BWI76_RS14415: BWI76_RS14415 - transcriptional regulator, TetR family protein, at 3,010,086 to 3,010,682
_RS14415
Position (kb)
3008
3009
3010 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3007.860 kb on + strand at 3008.043 kb on + strand at 3008.043 kb on + strand at 3008.045 kb on + strand at 3008.045 kb on + strand at 3008.077 kb on - strand at 3008.160 kb on - strand at 3008.247 kb on + strand at 3008.260 kb on + strand at 3008.260 kb on + strand at 3008.605 kb on - strand at 3008.909 kb on + strand, within BWI76_RS14400 at 3008.909 kb on + strand, within BWI76_RS14400 at 3008.959 kb on - strand, within BWI76_RS14400 at 3009.019 kb on - strand, within BWI76_RS14400 at 3009.084 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.096 kb on + strand, within BWI76_RS14400 at 3009.130 kb on - strand, within BWI76_RS14400 at 3009.130 kb on - strand, within BWI76_RS14400 at 3009.178 kb on + strand at 3009.178 kb on + strand at 3009.178 kb on + strand at 3009.251 kb on + strand at 3009.264 kb on - strand at 3009.766 kb on - strand at 3009.812 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.822 kb on - strand, within BWI76_RS14410 at 3009.848 kb on + strand, within BWI76_RS14410 at 3009.856 kb on - strand at 3009.962 kb on + strand at 3009.970 kb on - strand at 3009.970 kb on - strand at 3009.986 kb on + strand at 3009.989 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3009.994 kb on - strand at 3010.128 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 3,007,860 + -0.4 3,008,043 + +1.6 3,008,043 + +1.4 3,008,045 + -1.0 3,008,045 + +0.5 3,008,077 - -0.8 3,008,160 - +2.4 3,008,247 + -0.5 3,008,260 + +1.6 3,008,260 + +0.1 3,008,605 - -0.6 3,008,909 + BWI76_RS14400 0.49 -0.1 3,008,909 + BWI76_RS14400 0.49 +0.4 3,008,959 - BWI76_RS14400 0.58 -0.2 3,009,019 - BWI76_RS14400 0.68 -0.2 3,009,084 + BWI76_RS14400 0.80 -0.5 3,009,096 + BWI76_RS14400 0.82 -0.6 3,009,096 + BWI76_RS14400 0.82 -0.9 3,009,096 + BWI76_RS14400 0.82 -1.9 3,009,096 + BWI76_RS14400 0.82 +0.1 3,009,096 + BWI76_RS14400 0.82 -1.2 3,009,130 - BWI76_RS14400 0.88 +1.2 3,009,130 - BWI76_RS14400 0.88 -2.2 3,009,178 + -0.8 3,009,178 + +0.1 3,009,178 + -0.7 3,009,251 + +0.1 3,009,264 - +0.2 3,009,766 - +0.1 3,009,812 - BWI76_RS14410 0.24 +1.7 3,009,822 - BWI76_RS14410 0.28 -0.6 3,009,822 - BWI76_RS14410 0.28 -0.8 3,009,822 - BWI76_RS14410 0.28 +0.7 3,009,848 + BWI76_RS14410 0.41 -0.8 3,009,856 - +0.1 3,009,962 + -1.5 3,009,970 - -1.2 3,009,970 - +0.1 3,009,986 + -0.4 3,009,989 - +0.8 3,009,994 - -1.5 3,009,994 - -0.6 3,009,994 - -0.0 3,009,994 - +0.6 3,010,128 + -1.0
Or see this region's nucleotide sequence