Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14235 and BWI76_RS14240 are separated by 43 nucleotides BWI76_RS14240 and BWI76_RS14245 are separated by 35 nucleotides BWI76_RS14245 and BWI76_RS14250 are separated by 111 nucleotides
BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327
_RS14235
BWI76_RS14240: BWI76_RS14240 - thiol reductase thioredoxin, at 2,979,371 to 2,979,694
_RS14240
BWI76_RS14245: BWI76_RS14245 - carboxymuconolactone decarboxylase family protein, at 2,979,730 to 2,980,251
_RS14245
BWI76_RS14250: BWI76_RS14250 - cytochrome ubiquinol oxidase subunit I, at 2,980,363 to 2,981,781
_RS14250
Position (kb)
2979
2980
2981 Strain fitness (log2 ratio)
-2
-1
0
1 at 2978.744 kb on + strand, within BWI76_RS14235 at 2978.892 kb on + strand, within BWI76_RS14235 at 2978.900 kb on - strand, within BWI76_RS14235 at 2978.940 kb on + strand, within BWI76_RS14235 at 2979.367 kb on + strand at 2979.468 kb on - strand, within BWI76_RS14240 at 2979.563 kb on - strand, within BWI76_RS14240 at 2979.626 kb on + strand, within BWI76_RS14240 at 2979.634 kb on - strand, within BWI76_RS14240 at 2979.743 kb on + strand at 2979.796 kb on + strand, within BWI76_RS14245 at 2979.926 kb on + strand, within BWI76_RS14245 at 2979.943 kb on - strand, within BWI76_RS14245 at 2979.990 kb on - strand, within BWI76_RS14245 at 2980.261 kb on + strand at 2980.330 kb on - strand at 2980.581 kb on + strand at 2980.589 kb on - strand, within BWI76_RS14250 at 2980.703 kb on + strand, within BWI76_RS14250 at 2980.711 kb on - strand, within BWI76_RS14250 at 2980.778 kb on + strand, within BWI76_RS14250 at 2980.851 kb on - strand, within BWI76_RS14250 at 2980.870 kb on - strand, within BWI76_RS14250 at 2980.872 kb on - strand, within BWI76_RS14250 at 2980.875 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.918 kb on + strand, within BWI76_RS14250 at 2980.926 kb on - strand, within BWI76_RS14250 at 2980.935 kb on - strand, within BWI76_RS14250 at 2981.037 kb on - strand, within BWI76_RS14250 at 2981.158 kb on - strand, within BWI76_RS14250
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 2,978,744 + BWI76_RS14235 0.43 -0.7 2,978,892 + BWI76_RS14235 0.57 -0.4 2,978,900 - BWI76_RS14235 0.58 +0.3 2,978,940 + BWI76_RS14235 0.62 -0.5 2,979,367 + +0.2 2,979,468 - BWI76_RS14240 0.30 -1.5 2,979,563 - BWI76_RS14240 0.59 +0.1 2,979,626 + BWI76_RS14240 0.79 -0.9 2,979,634 - BWI76_RS14240 0.81 -0.7 2,979,743 + +0.0 2,979,796 + BWI76_RS14245 0.13 -0.8 2,979,926 + BWI76_RS14245 0.38 -0.5 2,979,943 - BWI76_RS14245 0.41 -0.5 2,979,990 - BWI76_RS14245 0.50 -2.0 2,980,261 + -0.2 2,980,330 - -1.3 2,980,581 + -0.4 2,980,589 - BWI76_RS14250 0.16 +0.5 2,980,703 + BWI76_RS14250 0.24 -0.9 2,980,711 - BWI76_RS14250 0.25 -0.8 2,980,778 + BWI76_RS14250 0.29 -1.5 2,980,851 - BWI76_RS14250 0.34 -0.2 2,980,870 - BWI76_RS14250 0.36 -0.1 2,980,872 - BWI76_RS14250 0.36 -0.1 2,980,875 + BWI76_RS14250 0.36 -0.2 2,980,918 + BWI76_RS14250 0.39 -0.1 2,980,918 + BWI76_RS14250 0.39 +0.8 2,980,926 - BWI76_RS14250 0.40 -0.5 2,980,935 - BWI76_RS14250 0.40 +0.1 2,981,037 - BWI76_RS14250 0.47 -1.2 2,981,158 - BWI76_RS14250 0.56 +0.7
Or see this region's nucleotide sequence