Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14230

Experiment: KLEB25 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14220 and BWI76_RS14225 are separated by 53 nucleotidesBWI76_RS14225 and BWI76_RS14230 overlap by 1 nucleotidesBWI76_RS14230 and BWI76_RS14235 are separated by 84 nucleotides BWI76_RS14220: BWI76_RS14220 - hypothetical protein, at 2,976,163 to 2,976,504 _RS14220 BWI76_RS14225: BWI76_RS14225 - anti-ECF sigma factor ChrR, at 2,976,558 to 2,977,229 _RS14225 BWI76_RS14230: BWI76_RS14230 - flavin monoamine oxidase family protein, at 2,977,229 to 2,978,218 _RS14230 BWI76_RS14235: BWI76_RS14235 - alcohol dehydrogenase, at 2,978,303 to 2,979,327 _RS14235 Position (kb) 2977 2978 2979Strain fitness (log2 ratio) -1 0 1at 2976.672 kb on + strand, within BWI76_RS14225at 2976.680 kb on - strand, within BWI76_RS14225at 2977.381 kb on + strand, within BWI76_RS14230at 2977.774 kb on + strand, within BWI76_RS14230at 2977.774 kb on + strand, within BWI76_RS14230at 2977.818 kb on - strand, within BWI76_RS14230at 2977.994 kb on + strand, within BWI76_RS14230at 2978.403 kb on - strandat 2978.449 kb on - strand, within BWI76_RS14235at 2978.546 kb on - strand, within BWI76_RS14235at 2978.552 kb on - strand, within BWI76_RS14235at 2978.552 kb on - strand, within BWI76_RS14235at 2978.702 kb on - strand, within BWI76_RS14235at 2978.744 kb on + strand, within BWI76_RS14235at 2978.892 kb on + strand, within BWI76_RS14235at 2978.900 kb on - strand, within BWI76_RS14235at 2978.940 kb on + strand, within BWI76_RS14235

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB25 MOI 1
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2,976,672 + BWI76_RS14225 0.17 +0.2
2,976,680 - BWI76_RS14225 0.18 -0.0
2,977,381 + BWI76_RS14230 0.15 -0.4
2,977,774 + BWI76_RS14230 0.55 -0.9
2,977,774 + BWI76_RS14230 0.55 -0.2
2,977,818 - BWI76_RS14230 0.59 -0.4
2,977,994 + BWI76_RS14230 0.77 -1.7
2,978,403 - -0.5
2,978,449 - BWI76_RS14235 0.14 -1.7
2,978,546 - BWI76_RS14235 0.24 +0.2
2,978,552 - BWI76_RS14235 0.24 -1.2
2,978,552 - BWI76_RS14235 0.24 +0.2
2,978,702 - BWI76_RS14235 0.39 +0.5
2,978,744 + BWI76_RS14235 0.43 -0.7
2,978,892 + BWI76_RS14235 0.57 -0.4
2,978,900 - BWI76_RS14235 0.58 +0.3
2,978,940 + BWI76_RS14235 0.62 -0.5

Or see this region's nucleotide sequence