Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13830 and BWI76_RS13835 are separated by 184 nucleotides BWI76_RS13835 and BWI76_RS13840 are separated by 236 nucleotides BWI76_RS13840 and BWI76_RS13845 are separated by 114 nucleotides
BWI76_RS13830: BWI76_RS13830 - NAD-dependent malic enzyme, at 2,885,110 to 2,886,807
_RS13830
BWI76_RS13835: BWI76_RS13835 - 30S ribosomal protein S22, at 2,886,992 to 2,887,132
_RS13835
BWI76_RS13840: BWI76_RS13840 - MBL fold metallo-hydrolase, at 2,887,369 to 2,888,220
_RS13840
BWI76_RS13845: BWI76_RS13845 - LysR family transcriptional regulator, at 2,888,335 to 2,889,237
_RS13845
Position (kb)
2887
2888
2889 Strain fitness (log2 ratio)
-2
-1
0
1 at 2886.500 kb on - strand, within BWI76_RS13830 at 2886.526 kb on + strand, within BWI76_RS13830 at 2886.539 kb on + strand, within BWI76_RS13830 at 2886.702 kb on + strand at 2886.715 kb on + strand at 2886.750 kb on + strand at 2886.760 kb on + strand at 2886.761 kb on + strand at 2886.877 kb on - strand at 2886.886 kb on + strand at 2886.886 kb on + strand at 2886.886 kb on + strand at 2886.894 kb on - strand at 2886.894 kb on - strand at 2886.909 kb on - strand at 2887.140 kb on + strand at 2887.226 kb on + strand at 2887.327 kb on - strand at 2887.425 kb on - strand at 2887.853 kb on + strand, within BWI76_RS13840 at 2887.917 kb on - strand, within BWI76_RS13840 at 2888.086 kb on - strand, within BWI76_RS13840 at 2888.096 kb on - strand, within BWI76_RS13840 at 2888.096 kb on - strand, within BWI76_RS13840 at 2888.177 kb on + strand at 2888.177 kb on + strand at 2888.340 kb on - strand at 2888.441 kb on + strand, within BWI76_RS13845 at 2888.468 kb on + strand, within BWI76_RS13845 at 2888.608 kb on - strand, within BWI76_RS13845 at 2888.681 kb on + strand, within BWI76_RS13845 at 2888.820 kb on + strand, within BWI76_RS13845 at 2888.820 kb on + strand, within BWI76_RS13845
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 2,886,500 - BWI76_RS13830 0.82 -2.2 2,886,526 + BWI76_RS13830 0.83 -0.7 2,886,539 + BWI76_RS13830 0.84 -0.6 2,886,702 + -0.0 2,886,715 + -0.4 2,886,750 + -0.3 2,886,760 + -0.9 2,886,761 + -0.7 2,886,877 - +0.8 2,886,886 + -0.1 2,886,886 + +0.3 2,886,886 + -0.9 2,886,894 - +0.1 2,886,894 - -0.8 2,886,909 - -0.7 2,887,140 + -0.2 2,887,226 + -0.4 2,887,327 - +0.4 2,887,425 - +0.0 2,887,853 + BWI76_RS13840 0.57 -1.0 2,887,917 - BWI76_RS13840 0.64 -1.1 2,888,086 - BWI76_RS13840 0.84 -1.1 2,888,096 - BWI76_RS13840 0.85 +0.4 2,888,096 - BWI76_RS13840 0.85 -0.4 2,888,177 + -0.1 2,888,177 + -0.1 2,888,340 - -0.6 2,888,441 + BWI76_RS13845 0.12 -0.7 2,888,468 + BWI76_RS13845 0.15 +0.0 2,888,608 - BWI76_RS13845 0.30 -1.4 2,888,681 + BWI76_RS13845 0.38 +0.8 2,888,820 + BWI76_RS13845 0.54 +0.7 2,888,820 + BWI76_RS13845 0.54 -1.0
Or see this region's nucleotide sequence