Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13300

Experiment: KLEB25 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13295 and BWI76_RS13300 are separated by 42 nucleotidesBWI76_RS13300 and BWI76_RS13305 are separated by 172 nucleotides BWI76_RS13295: BWI76_RS13295 - DUF1338 domain-containing protein, at 2,771,598 to 2,772,941 _RS13295 BWI76_RS13300: BWI76_RS13300 - carboxylesterase, at 2,772,984 to 2,774,492 _RS13300 BWI76_RS13305: BWI76_RS13305 - hydrolase, at 2,774,665 to 2,775,288 _RS13305 Position (kb) 2772 2773 2774 2775Strain fitness (log2 ratio) -2 -1 0 1at 2772.295 kb on + strand, within BWI76_RS13295at 2772.445 kb on + strand, within BWI76_RS13295at 2772.476 kb on - strand, within BWI76_RS13295at 2772.518 kb on - strand, within BWI76_RS13295at 2772.518 kb on - strand, within BWI76_RS13295at 2772.594 kb on + strand, within BWI76_RS13295at 2773.015 kb on - strandat 2773.167 kb on + strand, within BWI76_RS13300at 2773.240 kb on + strand, within BWI76_RS13300at 2773.367 kb on - strandat 2773.727 kb on + strand, within BWI76_RS13300at 2774.106 kb on + strand, within BWI76_RS13300at 2774.508 kb on + strandat 2775.271 kb on - strandat 2775.312 kb on - strandat 2775.448 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB25 MOI 1
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2,772,295 + BWI76_RS13295 0.52 +0.6
2,772,445 + BWI76_RS13295 0.63 +0.1
2,772,476 - BWI76_RS13295 0.65 +0.4
2,772,518 - BWI76_RS13295 0.68 -0.5
2,772,518 - BWI76_RS13295 0.68 +0.2
2,772,594 + BWI76_RS13295 0.74 -0.2
2,773,015 - -0.7
2,773,167 + BWI76_RS13300 0.12 -2.3
2,773,240 + BWI76_RS13300 0.17 -0.5
2,773,367 - +0.0
2,773,727 + BWI76_RS13300 0.49 -1.7
2,774,106 + BWI76_RS13300 0.74 -0.2
2,774,508 + +0.1
2,775,271 - +0.0
2,775,312 - -0.4
2,775,448 - -0.7

Or see this region's nucleotide sequence