Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13020 and BWI76_RS13025 are separated by 137 nucleotides BWI76_RS13025 and BWI76_RS13030 are separated by 214 nucleotides BWI76_RS13030 and BWI76_RS13035 are separated by 228 nucleotides
BWI76_RS13020: BWI76_RS13020 - NmrA family protein, at 2,716,832 to 2,717,821
_RS13020
BWI76_RS13025: BWI76_RS13025 - histidine phosphatase family protein, at 2,717,959 to 2,718,579
_RS13025
BWI76_RS13030: BWI76_RS13030 - site-specific recombinase, phage integrase family protein, at 2,718,794 to 2,719,396
_RS13030
BWI76_RS13035: BWI76_RS13035 - MFS transporter, at 2,719,625 to 2,721,079
_RS13035
Position (kb)
2718
2719
2720 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2718.049 kb on + strand, within BWI76_RS13025 at 2718.294 kb on + strand, within BWI76_RS13025 at 2718.419 kb on + strand, within BWI76_RS13025 at 2718.503 kb on + strand, within BWI76_RS13025 at 2718.633 kb on + strand at 2718.776 kb on + strand at 2719.149 kb on + strand, within BWI76_RS13030 at 2719.174 kb on + strand, within BWI76_RS13030 at 2719.460 kb on + strand at 2719.604 kb on - strand at 2719.681 kb on - strand at 2719.692 kb on + strand at 2719.737 kb on + strand at 2719.737 kb on + strand at 2719.745 kb on - strand at 2719.745 kb on - strand at 2719.745 kb on - strand at 2719.747 kb on + strand at 2719.799 kb on + strand, within BWI76_RS13035 at 2719.807 kb on - strand, within BWI76_RS13035 at 2719.835 kb on - strand, within BWI76_RS13035 at 2720.016 kb on - strand, within BWI76_RS13035 at 2720.018 kb on + strand, within BWI76_RS13035 at 2720.026 kb on - strand, within BWI76_RS13035 at 2720.043 kb on + strand, within BWI76_RS13035 at 2720.046 kb on - strand, within BWI76_RS13035 at 2720.089 kb on + strand, within BWI76_RS13035 at 2720.108 kb on - strand, within BWI76_RS13035 at 2720.145 kb on - strand, within BWI76_RS13035 at 2720.148 kb on + strand, within BWI76_RS13035 at 2720.199 kb on + strand, within BWI76_RS13035 at 2720.226 kb on - strand, within BWI76_RS13035 at 2720.226 kb on - strand, within BWI76_RS13035 at 2720.354 kb on + strand, within BWI76_RS13035 at 2720.354 kb on + strand, within BWI76_RS13035 at 2720.369 kb on + strand, within BWI76_RS13035 at 2720.377 kb on - strand at 2720.377 kb on - strand, within BWI76_RS13035
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 2,718,049 + BWI76_RS13025 0.14 -0.3 2,718,294 + BWI76_RS13025 0.54 +0.3 2,718,419 + BWI76_RS13025 0.74 -1.2 2,718,503 + BWI76_RS13025 0.88 -0.6 2,718,633 + +0.3 2,718,776 + +0.3 2,719,149 + BWI76_RS13030 0.59 -0.6 2,719,174 + BWI76_RS13030 0.63 -0.8 2,719,460 + -0.9 2,719,604 - -0.2 2,719,681 - -0.7 2,719,692 + -0.1 2,719,737 + -0.5 2,719,737 + +2.3 2,719,745 - -0.0 2,719,745 - -0.6 2,719,745 - -0.6 2,719,747 + -1.3 2,719,799 + BWI76_RS13035 0.12 -0.3 2,719,807 - BWI76_RS13035 0.13 +0.1 2,719,835 - BWI76_RS13035 0.14 +1.0 2,720,016 - BWI76_RS13035 0.27 +0.9 2,720,018 + BWI76_RS13035 0.27 -1.2 2,720,026 - BWI76_RS13035 0.28 -1.8 2,720,043 + BWI76_RS13035 0.29 +0.3 2,720,046 - BWI76_RS13035 0.29 -2.7 2,720,089 + BWI76_RS13035 0.32 +0.1 2,720,108 - BWI76_RS13035 0.33 +0.1 2,720,145 - BWI76_RS13035 0.36 -0.7 2,720,148 + BWI76_RS13035 0.36 -0.6 2,720,199 + BWI76_RS13035 0.39 -0.9 2,720,226 - BWI76_RS13035 0.41 +0.4 2,720,226 - BWI76_RS13035 0.41 -0.5 2,720,354 + BWI76_RS13035 0.50 -0.1 2,720,354 + BWI76_RS13035 0.50 +0.2 2,720,369 + BWI76_RS13035 0.51 -0.1 2,720,377 - +3.1 2,720,377 - BWI76_RS13035 0.52 -0.6
Or see this region's nucleotide sequence