Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10730 and BWI76_RS10735 are separated by 147 nucleotides BWI76_RS10735 and BWI76_RS10740 are separated by 19 nucleotides BWI76_RS10740 and BWI76_RS10745 are separated by 374 nucleotides
BWI76_RS10730: BWI76_RS10730 - bifunctional glucose-1-phosphatase/inositol phosphatase, at 2,267,463 to 2,268,710
_RS10730
BWI76_RS10735: BWI76_RS10735 - hypothetical protein, at 2,268,858 to 2,269,085
_RS10735
BWI76_RS10740: BWI76_RS10740 - NAD(P)H:quinone oxidoreductase, at 2,269,105 to 2,269,701
_RS10740
BWI76_RS10745: BWI76_RS10745 - hypothetical protein, at 2,270,076 to 2,270,249
_RS10745
Position (kb)
2268
2269
2270 Strain fitness (log2 ratio)
-1
0
1 at 2267.861 kb on - strand, within BWI76_RS10730 at 2268.911 kb on - strand, within BWI76_RS10735 at 2269.145 kb on + strand at 2269.145 kb on + strand at 2269.610 kb on - strand, within BWI76_RS10740 at 2269.634 kb on - strand, within BWI76_RS10740 at 2269.652 kb on + strand at 2269.881 kb on - strand at 2269.988 kb on - strand at 2270.016 kb on + strand at 2270.024 kb on - strand at 2270.035 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 2,267,861 - BWI76_RS10730 0.32 +0.4 2,268,911 - BWI76_RS10735 0.23 -1.1 2,269,145 + +1.3 2,269,145 + +0.0 2,269,610 - BWI76_RS10740 0.85 +0.2 2,269,634 - BWI76_RS10740 0.89 -0.3 2,269,652 + -0.6 2,269,881 - -0.7 2,269,988 - -0.0 2,270,016 + +0.6 2,270,024 - +0.2 2,270,035 + -1.6
Or see this region's nucleotide sequence