Experiment: KLEB25 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00410 and BWI76_RS00415 are separated by 133 nucleotides BWI76_RS00415 and BWI76_RS00420 overlap by 7 nucleotides BWI76_RS00420 and BWI76_RS00425 overlap by 4 nucleotides BWI76_RS00425 and BWI76_RS00430 are separated by 44 nucleotides
BWI76_RS00410: BWI76_RS00410 - translational GTPase TypA, at 85,322 to 87,145
_RS00410
BWI76_RS00415: BWI76_RS00415 - glucose-1-phosphatase, at 87,279 to 87,878
_RS00415
BWI76_RS00420: BWI76_RS00420 - hypothetical protein, at 87,872 to 88,732
_RS00420
BWI76_RS00425: BWI76_RS00425 - D-tyrosyl-tRNA(Tyr) deacylase, at 88,729 to 89,166
_RS00425
BWI76_RS00430: BWI76_RS00430 - GNAT family N-acetyltransferase, at 89,211 to 90,152
_RS00430
Position (kb) 87
88
89 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 87.087 kb on + strand at 87.269 kb on - strand at 87.414 kb on - strand, within BWI76_RS00415 at 87.492 kb on + strand, within BWI76_RS00415 at 87.523 kb on + strand, within BWI76_RS00415 at 87.543 kb on - strand, within BWI76_RS00415 at 87.604 kb on - strand, within BWI76_RS00415 at 87.614 kb on + strand, within BWI76_RS00415 at 87.772 kb on - strand, within BWI76_RS00415 at 87.796 kb on + strand, within BWI76_RS00415 at 87.804 kb on - strand, within BWI76_RS00415 at 87.882 kb on - strand at 88.062 kb on + strand, within BWI76_RS00420 at 88.140 kb on + strand, within BWI76_RS00420 at 88.295 kb on - strand, within BWI76_RS00420 at 88.316 kb on - strand, within BWI76_RS00420 at 88.464 kb on + strand, within BWI76_RS00420 at 88.464 kb on - strand, within BWI76_RS00420 at 88.472 kb on - strand, within BWI76_RS00420 at 88.484 kb on - strand, within BWI76_RS00420 at 88.523 kb on + strand, within BWI76_RS00420 at 88.531 kb on - strand, within BWI76_RS00420 at 88.599 kb on - strand, within BWI76_RS00420 at 88.648 kb on + strand at 88.736 kb on + strand at 89.025 kb on + strand, within BWI76_RS00425 at 89.025 kb on + strand, within BWI76_RS00425 at 89.046 kb on - strand, within BWI76_RS00425 at 89.216 kb on - strand at 89.390 kb on + strand, within BWI76_RS00430 at 89.453 kb on + strand, within BWI76_RS00430 at 89.461 kb on - strand, within BWI76_RS00430 at 89.638 kb on + strand, within BWI76_RS00430
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB25 MOI 1 remove 87,087 + -1.4 87,269 - -0.7 87,414 - BWI76_RS00415 0.23 -0.1 87,492 + BWI76_RS00415 0.35 -0.3 87,523 + BWI76_RS00415 0.41 -0.0 87,543 - BWI76_RS00415 0.44 +1.2 87,604 - BWI76_RS00415 0.54 -4.2 87,614 + BWI76_RS00415 0.56 -0.0 87,772 - BWI76_RS00415 0.82 -0.1 87,796 + BWI76_RS00415 0.86 -0.5 87,804 - BWI76_RS00415 0.88 -3.0 87,882 - -1.1 88,062 + BWI76_RS00420 0.22 -1.8 88,140 + BWI76_RS00420 0.31 -1.1 88,295 - BWI76_RS00420 0.49 -2.0 88,316 - BWI76_RS00420 0.52 -0.8 88,464 + BWI76_RS00420 0.69 +0.3 88,464 - BWI76_RS00420 0.69 -1.1 88,472 - BWI76_RS00420 0.70 -1.6 88,484 - BWI76_RS00420 0.71 +0.1 88,523 + BWI76_RS00420 0.76 -0.0 88,531 - BWI76_RS00420 0.77 +0.2 88,599 - BWI76_RS00420 0.84 -1.1 88,648 + -0.0 88,736 + +0.5 89,025 + BWI76_RS00425 0.68 -0.0 89,025 + BWI76_RS00425 0.68 +0.3 89,046 - BWI76_RS00425 0.72 +0.1 89,216 - -0.8 89,390 + BWI76_RS00430 0.19 +0.0 89,453 + BWI76_RS00430 0.26 -1.7 89,461 - BWI76_RS00430 0.27 -0.9 89,638 + BWI76_RS00430 0.45 -0.4
Or see this region's nucleotide sequence