Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20890 and BWI76_RS20895 are separated by 120 nucleotides BWI76_RS20895 and BWI76_RS20900 are separated by 108 nucleotides BWI76_RS20900 and BWI76_RS20905 are separated by 16 nucleotides
BWI76_RS20890: BWI76_RS20890 - neutral zinc metallopeptidase, at 4,368,605 to 4,369,474
_RS20890
BWI76_RS20895: BWI76_RS20895 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 4,369,595 to 4,370,308
_RS20895
BWI76_RS20900: BWI76_RS20900 - outer membrane protein assembly factor BamC, at 4,370,417 to 4,371,451
_RS20900
BWI76_RS20905: BWI76_RS20905 - 4-hydroxy-tetrahydrodipicolinate synthase, at 4,371,468 to 4,372,346
_RS20905
Position (kb)
4370
4371
4372 Strain fitness (log2 ratio)
-1
0
1 at 4369.646 kb on - strand at 4369.705 kb on - strand, within BWI76_RS20895 at 4369.745 kb on - strand, within BWI76_RS20895 at 4369.755 kb on + strand, within BWI76_RS20895 at 4369.755 kb on + strand, within BWI76_RS20895 at 4369.755 kb on + strand, within BWI76_RS20895 at 4370.032 kb on - strand, within BWI76_RS20895 at 4370.095 kb on + strand, within BWI76_RS20895 at 4370.460 kb on + strand at 4370.759 kb on - strand, within BWI76_RS20900 at 4370.981 kb on + strand, within BWI76_RS20900 at 4371.449 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 4,369,646 - +0.8 4,369,705 - BWI76_RS20895 0.15 +0.1 4,369,745 - BWI76_RS20895 0.21 +0.7 4,369,755 + BWI76_RS20895 0.22 -0.1 4,369,755 + BWI76_RS20895 0.22 +0.4 4,369,755 + BWI76_RS20895 0.22 -0.5 4,370,032 - BWI76_RS20895 0.61 -0.2 4,370,095 + BWI76_RS20895 0.70 -0.2 4,370,460 + +1.0 4,370,759 - BWI76_RS20900 0.33 -0.6 4,370,981 + BWI76_RS20900 0.54 -0.2 4,371,449 - +0.4
Or see this region's nucleotide sequence