Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18190

Experiment: KLEB15 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18180 and BWI76_RS18185 are separated by 53 nucleotidesBWI76_RS18185 and BWI76_RS18190 are separated by 39 nucleotidesBWI76_RS18190 and BWI76_RS18195 overlap by 4 nucleotides BWI76_RS18180: BWI76_RS18180 - hypothetical protein, at 3,777,514 to 3,778,332 _RS18180 BWI76_RS18185: BWI76_RS18185 - membrane protein, at 3,778,386 to 3,778,781 _RS18185 BWI76_RS18190: BWI76_RS18190 - carboxy-S-adenosyl-L-methionine synthase CmoA, at 3,778,821 to 3,779,564 _RS18190 BWI76_RS18195: BWI76_RS18195 - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 3,779,561 to 3,780,577 _RS18195 Position (kb) 3778 3779 3780Strain fitness (log2 ratio) -1 0 1 2at 3777.930 kb on + strand, within BWI76_RS18180at 3777.977 kb on - strand, within BWI76_RS18180at 3778.454 kb on + strand, within BWI76_RS18185at 3778.478 kb on - strand, within BWI76_RS18185at 3778.484 kb on - strand, within BWI76_RS18185at 3778.603 kb on - strand, within BWI76_RS18185at 3778.605 kb on + strand, within BWI76_RS18185at 3778.649 kb on + strand, within BWI76_RS18185at 3778.744 kb on + strandat 3778.752 kb on - strandat 3779.110 kb on - strand, within BWI76_RS18190at 3779.150 kb on + strand, within BWI76_RS18190at 3779.775 kb on + strand, within BWI76_RS18195at 3779.814 kb on + strand, within BWI76_RS18195at 3780.107 kb on - strand, within BWI76_RS18195at 3780.317 kb on - strand, within BWI76_RS18195

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1
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3,777,930 + BWI76_RS18180 0.51 +0.3
3,777,977 - BWI76_RS18180 0.57 -0.1
3,778,454 + BWI76_RS18185 0.17 -0.2
3,778,478 - BWI76_RS18185 0.23 -0.1
3,778,484 - BWI76_RS18185 0.25 -0.1
3,778,603 - BWI76_RS18185 0.55 +0.8
3,778,605 + BWI76_RS18185 0.55 +0.3
3,778,649 + BWI76_RS18185 0.66 +0.0
3,778,744 + +2.4
3,778,752 - -0.3
3,779,110 - BWI76_RS18190 0.39 -0.6
3,779,150 + BWI76_RS18190 0.44 -0.6
3,779,775 + BWI76_RS18195 0.21 -0.3
3,779,814 + BWI76_RS18195 0.25 -1.2
3,780,107 - BWI76_RS18195 0.54 +0.4
3,780,317 - BWI76_RS18195 0.74 +0.1

Or see this region's nucleotide sequence