Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17915 and BWI76_RS17920 are separated by 3 nucleotides BWI76_RS17920 and BWI76_RS17925 are separated by 60 nucleotides BWI76_RS17925 and BWI76_RS17930 are separated by 190 nucleotides BWI76_RS17930 and BWI76_RS17935 are separated by 41 nucleotides BWI76_RS17935 and BWI76_RS17940 overlap by 39 nucleotides
BWI76_RS17915: BWI76_RS17915 - membrane protein, at 3,725,209 to 3,725,775
_RS17915
BWI76_RS17920: BWI76_RS17920 - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 3,725,779 to 3,726,573
_RS17920
BWI76_RS17925: BWI76_RS17925 - peptidoglycan synthase, at 3,726,634 to 3,728,379
_RS17925
BWI76_RS17930: BWI76_RS17930 - cold-shock protein CspC, at 3,728,570 to 3,728,779
_RS17930
BWI76_RS17935: BWI76_RS17935 - DUF2627 domain-containing protein, at 3,728,821 to 3,728,937
_RS17935
BWI76_RS17940: BWI76_RS17940 - hypothetical protein, at 3,728,899 to 3,729,093
_RS17940
Position (kb)
3726
3727
3728
3729 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3725.728 kb on - strand at 3725.743 kb on + strand at 3725.926 kb on - strand, within BWI76_RS17920 at 3725.979 kb on + strand, within BWI76_RS17920 at 3726.603 kb on + strand at 3726.611 kb on - strand at 3726.993 kb on - strand, within BWI76_RS17925 at 3727.153 kb on + strand, within BWI76_RS17925 at 3727.353 kb on + strand, within BWI76_RS17925 at 3727.368 kb on - strand, within BWI76_RS17925 at 3727.649 kb on + strand, within BWI76_RS17925 at 3727.657 kb on - strand, within BWI76_RS17925 at 3727.723 kb on + strand, within BWI76_RS17925 at 3727.943 kb on - strand, within BWI76_RS17925 at 3727.967 kb on + strand, within BWI76_RS17925 at 3728.292 kb on + strand at 3728.333 kb on - strand at 3728.606 kb on - strand, within BWI76_RS17930 at 3728.684 kb on - strand, within BWI76_RS17930 at 3728.687 kb on - strand, within BWI76_RS17930 at 3728.733 kb on + strand, within BWI76_RS17930 at 3728.741 kb on - strand, within BWI76_RS17930 at 3728.763 kb on + strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.797 kb on - strand at 3728.826 kb on - strand at 3728.860 kb on + strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.913 kb on - strand at 3728.943 kb on + strand, within BWI76_RS17940 at 3728.943 kb on - strand, within BWI76_RS17940 at 3728.946 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.954 kb on - strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.041 kb on - strand, within BWI76_RS17940 at 3729.057 kb on + strand, within BWI76_RS17940 at 3729.156 kb on - strand at 3729.185 kb on - strand at 3729.202 kb on + strand at 3729.213 kb on + strand at 3729.235 kb on - strand at 3729.317 kb on + strand at 3729.317 kb on + strand at 3729.325 kb on - strand at 3729.352 kb on + strand at 3729.362 kb on + strand at 3729.362 kb on + strand at 3729.365 kb on + strand at 3729.370 kb on - strand at 3729.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 3,725,728 - -0.7 3,725,743 + +0.1 3,725,926 - BWI76_RS17920 0.18 -0.5 3,725,979 + BWI76_RS17920 0.25 -2.3 3,726,603 + +1.0 3,726,611 - -0.0 3,726,993 - BWI76_RS17925 0.21 -0.4 3,727,153 + BWI76_RS17925 0.30 -2.0 3,727,353 + BWI76_RS17925 0.41 -0.1 3,727,368 - BWI76_RS17925 0.42 -0.1 3,727,649 + BWI76_RS17925 0.58 -0.5 3,727,657 - BWI76_RS17925 0.59 -1.0 3,727,723 + BWI76_RS17925 0.62 -1.1 3,727,943 - BWI76_RS17925 0.75 +0.1 3,727,967 + BWI76_RS17925 0.76 -0.5 3,728,292 + -0.1 3,728,333 - -1.3 3,728,606 - BWI76_RS17930 0.17 -0.1 3,728,684 - BWI76_RS17930 0.54 -0.3 3,728,687 - BWI76_RS17930 0.56 -0.6 3,728,733 + BWI76_RS17930 0.78 +1.8 3,728,741 - BWI76_RS17930 0.81 -0.3 3,728,763 + -0.8 3,728,771 - +0.2 3,728,771 - -0.6 3,728,771 - -0.1 3,728,797 - -0.1 3,728,826 - -0.5 3,728,860 + BWI76_RS17935 0.33 -0.8 3,728,868 - BWI76_RS17935 0.40 -0.2 3,728,868 - BWI76_RS17935 0.40 -0.1 3,728,913 - -0.2 3,728,943 + BWI76_RS17940 0.23 -0.5 3,728,943 - BWI76_RS17940 0.23 -0.3 3,728,946 - BWI76_RS17940 0.24 -0.0 3,728,951 - BWI76_RS17940 0.27 -0.2 3,728,951 - BWI76_RS17940 0.27 -0.6 3,728,954 - BWI76_RS17940 0.28 -0.2 3,729,014 + BWI76_RS17940 0.59 -1.4 3,729,014 + BWI76_RS17940 0.59 -0.5 3,729,014 + BWI76_RS17940 0.59 +0.5 3,729,014 + BWI76_RS17940 0.59 -0.2 3,729,014 + +0.8 3,729,014 + BWI76_RS17940 0.59 -0.6 3,729,014 + BWI76_RS17940 0.59 +0.1 3,729,022 - BWI76_RS17940 0.63 -0.3 3,729,022 - BWI76_RS17940 0.63 -0.6 3,729,022 - BWI76_RS17940 0.63 -2.0 3,729,022 - BWI76_RS17940 0.63 -0.6 3,729,022 - BWI76_RS17940 0.63 -0.3 3,729,022 - BWI76_RS17940 0.63 -0.5 3,729,041 - BWI76_RS17940 0.73 -2.2 3,729,057 + BWI76_RS17940 0.81 +0.6 3,729,156 - -0.1 3,729,185 - -1.5 3,729,202 + +1.3 3,729,213 + +0.0 3,729,235 - +2.8 3,729,317 + -1.7 3,729,317 + -1.0 3,729,325 - -0.6 3,729,352 + -0.3 3,729,362 + -0.5 3,729,362 + +0.3 3,729,365 + +0.8 3,729,370 - -0.4 3,729,370 - +0.2
Or see this region's nucleotide sequence