Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16280

Experiment: KLEB15 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16275 and BWI76_RS16280 are separated by 197 nucleotidesBWI76_RS16280 and BWI76_RS16285 are separated by 98 nucleotides BWI76_RS16275: BWI76_RS16275 - fumarate hydratase FumA, at 3,376,683 to 3,378,329 _RS16275 BWI76_RS16280: BWI76_RS16280 - mannose-6-phosphate isomerase, at 3,378,527 to 3,379,705 _RS16280 BWI76_RS16285: BWI76_RS16285 - hypothetical protein, at 3,379,804 to 3,381,309 _RS16285 Position (kb) 3378 3379 3380Strain fitness (log2 ratio) -1 0 1at 3377.840 kb on + strand, within BWI76_RS16275at 3378.245 kb on + strandat 3378.253 kb on + strandat 3378.256 kb on - strandat 3378.326 kb on + strandat 3378.546 kb on - strandat 3378.658 kb on - strand, within BWI76_RS16280at 3378.812 kb on + strand, within BWI76_RS16280at 3378.916 kb on + strand, within BWI76_RS16280at 3379.465 kb on - strand, within BWI76_RS16280at 3379.537 kb on + strand, within BWI76_RS16280at 3379.933 kb on + strandat 3380.154 kb on + strand, within BWI76_RS16285at 3380.167 kb on - strand, within BWI76_RS16285at 3380.562 kb on + strand, within BWI76_RS16285at 3380.622 kb on - strand, within BWI76_RS16285

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1
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3,377,840 + BWI76_RS16275 0.70 -0.8
3,378,245 + +0.6
3,378,253 + -0.8
3,378,256 - -0.0
3,378,326 + +1.3
3,378,546 - -0.8
3,378,658 - BWI76_RS16280 0.11 -0.2
3,378,812 + BWI76_RS16280 0.24 -0.8
3,378,916 + BWI76_RS16280 0.33 -0.4
3,379,465 - BWI76_RS16280 0.80 -0.5
3,379,537 + BWI76_RS16280 0.86 -0.7
3,379,933 + -0.1
3,380,154 + BWI76_RS16285 0.23 -0.3
3,380,167 - BWI76_RS16285 0.24 +0.2
3,380,562 + BWI76_RS16285 0.50 +0.2
3,380,622 - BWI76_RS16285 0.54 +0.1

Or see this region's nucleotide sequence